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Antigenic Diversity of Human Sapoviruses
- Source :
- Emerging Infectious Diseases, Vol 13, Iss 10, Pp 1519-1525 (2007), Emerging Infectious Diseases
- Publication Year :
- 2007
- Publisher :
- Centers for Disease Control and Prevention (CDC), 2007.
-
Abstract
- Correspondence between virus antigenicity and capsid (VP1) genogrouping and genotyping is likely.<br />Sapovirus (SaV) is a causative agent of gastroenteritis. On the basis of capsid protein (VP1) nucleotide sequences, SaV can be divided into 5 genogroups (GI–GV), of which the GI, GII, GIV, and GV strains infect humans. SaV is uncultivable, but expression of recombinant VP1 in insect cells results in formation of viruslike particles (VLPs) that are antigenically similar to native SaV. In this study, we newly expressed SaV GII and GIV VLPs to compare genetic and antigenic relationships among all human SaV genogroups. Hyperimmune antiserum samples against VLPs reacted strongly with homologous VLPs. However, several antiserum samples weakly cross-reacted against heterologous VLPs in an antibody ELISA. Conversely, an antigen ELISA showed that VLPs of SaV in all human genogroups were antigenically distinct. These findings indicate a likely correspondence between SaV antigenicity and VP1 genogrouping and genotyping.
- Subjects :
- Microbiology (medical)
Antigenicity
cross-reactivity
Epidemiology
genotype
viruses
lcsh:Medicine
antiserum
virus-like particles
complex mixtures
Sapovirus
lcsh:Infectious and parasitic diseases
Microbiology
law.invention
Antigenic Diversity
fluids and secretions
stomatognathic system
Antigen
law
Humans
lcsh:RC109-216
genogroup
Phylogeny
Caliciviridae Infections
Antiserum
biology
Sequence Analysis, RNA
Research
lcsh:R
virus diseases
biochemical phenomena, metabolism, and nutrition
biology.organism_classification
Virology
capsid protein
Gastroenteritis
Infectious Diseases
Capsid
biology.protein
Recombinant DNA
Capsid Proteins
Antibody
Subjects
Details
- ISSN :
- 10806059 and 10806040
- Volume :
- 13
- Database :
- OpenAIRE
- Journal :
- Emerging Infectious Diseases
- Accession number :
- edsair.doi.dedup.....bf014bbff5bcf0d73c0d7421dbf11d4f
- Full Text :
- https://doi.org/10.3201/eid1310.070402