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Human multipotent adipose-derived stem cells differentiate into functional brown adipocytes
- Source :
- Elabd, C, Chiellini, C, Carmona, M, Galitzky, J, Cochet, O, Petersen, R, Pénicaud, L, Kristiansen, K, Bouloumié, A, Casteilla, L, Dani, C, Ailhaud, G & Amri, E-Z 2009, ' Human multipotent adipose-derived stem cells differentiate into functional brown adipocytes ', Stem Cells, vol. 27, no. 11, pp. 2753-60 . https://doi.org/10.1002/stem.200, Stem Cells / Stem Cells (Miamisburg), Stem Cells / Stem Cells (Miamisburg), Alphamed Press, 2009, epub ahead of print. ⟨10.1002/stem.200⟩, Stem Cells / Stem Cells (Miamisburg), 2009, epub ahead of print. ⟨10.1002/stem.200⟩
- Publication Year :
- 2009
-
Abstract
- In contrast to the earlier contention, adult humans have been shown recently to possess active brown adipose tissue with a potential of being of metabolic significance. Up to now, brown fat precursor cells have not been available for human studies. We have shown previously that human multipotent adipose-derived stem (hMADS) cells exhibit a normal karyotype and high self-renewal ability; they are known to differentiate into cells that exhibit the key properties of human white adipocytes, that is, uncoupling protein two expression, insulin-stimulated glucose uptake, lipolysis in response to β-agonists and atrial natriuretic peptide, and release of adiponectin and leptin. Herein, we show that, upon chronic exposure to a specific PPARγ but not to a PPARβ/δ or a PPARα agonist, hMADS cell-derived white adipocytes are able to switch to a brown phenotype by expressing both uncoupling protein one (UCP1) and CIDEA mRNA. This switch is accompanied by an increase in oxygen consumption and uncoupling. The expression of UCP1 protein is associated to stimulation of respiration by β-AR agonists, including β3-AR agonist. Thus, hMADS cells represent an invaluable cell model to screen for drugs stimulating the formation and/or the uncoupling capacity of human brown adipocytes that could help to dissipate excess caloric intake of individuals. Disclosure of potential conflicts of interest is found at the end of this article.
- Subjects :
- Male
medicine.medical_specialty
Adipose Tissue, White
Blotting, Western
Cell Respiration
Adipose tissue
030209 endocrinology & metabolism
White adipose tissue
Biology
Ion Channels
Cell Line
Mitochondrial Proteins
Rosiglitazone
03 medical and health sciences
chemistry.chemical_compound
Oxygen Consumption
0302 clinical medicine
Adipocyte
Internal medicine
Brown adipose tissue
medicine
Humans
Uncoupling protein
Cells, Cultured
Uncoupling Protein 1
030304 developmental biology
PRDM16
0303 health sciences
Reverse Transcriptase Polymerase Chain Reaction
Multipotent Stem Cells
Cell Differentiation
Cell Biology
Thermogenin
Adipocytes, Brown
Endocrinology
medicine.anatomical_structure
chemistry
Child, Preschool
Receptors, Adrenergic, beta-3
Androgens
Molecular Medicine
Thiazolidinediones
Stem cell
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 15494918
- Database :
- OpenAIRE
- Journal :
- Elabd, C, Chiellini, C, Carmona, M, Galitzky, J, Cochet, O, Petersen, R, Pénicaud, L, Kristiansen, K, Bouloumié, A, Casteilla, L, Dani, C, Ailhaud, G & Amri, E-Z 2009, ' Human multipotent adipose-derived stem cells differentiate into functional brown adipocytes ', Stem Cells, vol. 27, no. 11, pp. 2753-60 . https://doi.org/10.1002/stem.200, Stem Cells / Stem Cells (Miamisburg), Stem Cells / Stem Cells (Miamisburg), Alphamed Press, 2009, epub ahead of print. ⟨10.1002/stem.200⟩, Stem Cells / Stem Cells (Miamisburg), 2009, epub ahead of print. ⟨10.1002/stem.200⟩
- Accession number :
- edsair.doi.dedup.....bf47e33963b3369fd7bf931eac5e0ba7