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Evidence for N-7 guanine methyl transferase activity encoded within the modular domain of RNA-dependent RNA polymerase L of a Morbillivirus
- Source :
- IndraStra Global.
- Publication Year :
- 2015
- Publisher :
- SPRINGER, 2015.
-
Abstract
- Post-transcriptional modification of viral mRNA is essential for the translation of viral proteins by cellular translation machinery. Due to the cytoplasmic replication of Paramyxoviruses, the viral-encoded RNA-dependent RNA polymerase (RdRP) is thought to possess all activities required for mRNA capping and methylation. In the present work, using partially purified recombinant RNA polymerase complex of rinderpest virus expressed in insect cells, we demonstrate the in vitro methylation of capped mRNA. Further, we show that a recombinant C-terminal fragment (1717-2183 aa) of L protein is capable of methylating capped mRNA, suggesting that the various post-transcriptional activities of the L protein are located in independently folding domains.
- Subjects :
- Microbiology & Cell Biology
Messenger RNA
Five-prime cap
viruses
RNA-dependent RNA polymerase
Translation (biology)
General Medicine
Methylation
RNA polymerase complex
Biology
Molecular biology
law.invention
chemistry.chemical_compound
chemistry
law
Virology
RNA polymerase
Genetics
Recombinant DNA
Molecular Biology
Subjects
Details
- Language :
- English
- ISSN :
- 23813652
- Database :
- OpenAIRE
- Journal :
- IndraStra Global
- Accession number :
- edsair.doi.dedup.....bf62156ae597fe146c61d44ec90af801