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Islet amyloid polypeptide tonally inhibits β-, α-, and δ-cell secretion in isolated rat pancreatic islets
- Source :
- American Journal of Physiology-Endocrinology and Metabolism. 276:E19-E24
- Publication Year :
- 1999
- Publisher :
- American Physiological Society, 1999.
-
Abstract
- Islet amyloid polypeptide (IAPP, or amylin) is produced in pancreatic β-cells. The intraislet significance of IAPP is still uncertain. In the present study, paracrine effects of endogenous IAPP and somatostatin were investigated in isolated rat pancreatic islets. The intraislet IAPP activity was inhibited with an IAPP antiserum or a specific antagonist [IAPP-(8—37)]. Somatostatin activity was inhibited by immunoneutralization. Basal insulin and glucagon secretion were not affected by the somatostatin and/or IAPP blockade. Arginine-stimulated insulin and glucagon secretion were dose dependently increased by IAPP antiserum, IAPP-(8—37), and somatostatin antiserum, respectively. Arginine-stimulated somatostatin secretion was dose dependently potentiated by IAPP antiserum. Insulin secretion induced by 16.7 mM glucose was enhanced by IAPP antiserum and IAPP-(8—37), respectively. A combination of somatostatin antiserum with IAPP antiserum or IAPP-(8—37) further enhanced the arginine-stimulated insulin and glucagon secretion compared with effects when the blocking reagents were used individually. These results indicate that endogenously produced IAPP tonally inhibits stimulated insulin, glucagon, and somatostatin secretion. Furthermore, the paracrine effects of IAPP and somatostatin are additive.
- Subjects :
- Male
Amyloid
endocrine system
medicine.medical_specialty
Physiology
Endocrinology, Diabetes and Metabolism
Amylin
In Vitro Techniques
Glucagon
Rats, Sprague-Dawley
Islets of Langerhans
Paracrine signalling
Physiology (medical)
Internal medicine
Insulin Secretion
medicine
Animals
Insulin
Secretion
Pancreatic hormone
geography
geography.geographical_feature_category
Chemistry
Immune Sera
Pancreatic islets
Islet
Peptide Fragments
Islet Amyloid Polypeptide
Rats
Endocrinology
medicine.anatomical_structure
Somatostatin
hormones, hormone substitutes, and hormone antagonists
Subjects
Details
- ISSN :
- 15221555 and 01931849
- Volume :
- 276
- Database :
- OpenAIRE
- Journal :
- American Journal of Physiology-Endocrinology and Metabolism
- Accession number :
- edsair.doi.dedup.....bfe9ad8fe41b1631008ae3f92f786896