Back to Search
Start Over
Localization of steroidogenic enzymes and Foxl2a in the gonads of mature zebrafish [i](Danio rerio)[/i]
- Source :
- Comparative Biochemistry and Physiology-Part A: Molecular and Integrative Physiology, Comparative Biochemistry and Physiology-Part A: Molecular and Integrative Physiology, Elsevier, 2015, 188, pp.96-106. ⟨10.1016/j.cbpa.2015.06.016⟩
- Publication Year :
- 2015
- Publisher :
- HAL CCSD, 2015.
-
Abstract
- In zebrafish, the identification of the cells expressing steroidogenic enzymes and their regulators is far from completely fulfilled though it could provide crucial information on the elucidation of the role of these enzymes. The aim of this study was to better characterize the expression pattern of steroidogenic enzymes involved in estrogen and androgen production (Cyp17-I, Cyp11c1, Cyp19a1a and Cyp19a1b) and one of their regulators (Foxl2a) in zebrafish gonads. By using immunohistochemistry, we localized the steroid-producing cells in mature zebrafish gonads and determined different expression patterns between males and females. All these steroidogenic enzymes and Foxl2a were detected both in the testis and ovary. In the testis, they were all localized both in Leydig and germ cells except Cyp19a1b which was only detected in germ cells. In the ovary, Cyp17-I, Cyp19a1a and Foxl2a were immunolocalized in both somatic and germ cells while Cyp19a1b was only detected in germ cells and Cyp11c1 in somatic cells. Moreover, Cyp19a1a and Foxl2a did not display exactly the same patterns of spatial localization but their expressions were correlated suggesting a possible regulation of cyp19a1a gene by Foxl2a in zebrafish. Comparative analysis revealed a dimorphic expression of Cyp11c1, Cyp19a1a, Cyp19a1b and Foxl2a between males and females. Overall, our study provides a detailed description of the expression of proteins involved in the biosynthesis of steroidal hormones at the cellular scale within gonads, which is critical to further elucidating the intimate roles of the enzymes and the use of the zebrafish as a model in the field of endocrinology.
- Subjects :
- Forkhead Box Protein L2
Male
Physiology
Somatic cell
Steroidogenic enzymes
17-Alpha-hydroxylase
[SDV]Life Sciences [q-bio]
Danio
Ovary
Foxl2a
Biochemistry
Isozyme
03 medical and health sciences
Aromatases
0302 clinical medicine
Aromatase
Cytochrome P-450 Enzyme System
Testis
medicine
Animals
Gonads
Molecular Biology
Gene
Zebrafish
030304 developmental biology
0303 health sciences
biology
Steroid 17-alpha-Hydroxylase
Forkhead Transcription Factors
Zebrafish Proteins
biology.organism_classification
Molecular biology
Immunohistochemistry
Cell biology
Isoenzymes
20 lyase
medicine.anatomical_structure
Microscopy, Fluorescence
CYP17A1
biology.protein
Steroid 11-beta-Hydroxylase
Female
11-Beta-hydroxylase
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 10956433
- Database :
- OpenAIRE
- Journal :
- Comparative Biochemistry and Physiology-Part A: Molecular and Integrative Physiology, Comparative Biochemistry and Physiology-Part A: Molecular and Integrative Physiology, Elsevier, 2015, 188, pp.96-106. ⟨10.1016/j.cbpa.2015.06.016⟩
- Accession number :
- edsair.doi.dedup.....bff454d9efdf2713f92ebeb4bc85ef80