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A modified relief of unilateral ureteral obstruction model
- Source :
- Renal Failure, Renal Failure, Vol 41, Iss 1, Pp 497-506 (2019)
- Publication Year :
- 2019
- Publisher :
- Taylor & Francis, 2019.
-
Abstract
- Objectives: To improve the mouse model of relief for unilateral ureteral obstruction (RUUO) and explore the pathological process of renal fibrosis after the obstruction was relieved. Methods: C57BL/6 mice in model group were randomly divided into RUUO group, improved RUUO group, and UUO group. After leaving Unilateral Ureteral Obstruction (UUO) for 3 days, the obstruction was released by reimplantation way in RUUO group and in reimplantation + catheter way in improved RUUO group. C57BL/6 mice in observation group were randomly divided into 1d RUUO group, 3d RUUO group, 7d RUUO group, and 14d RUUO group. Three days after UUO, the obstruction was released by reimplantation + catheter in four groups. We detected the renal volume, H&E, Masson staining, and immunohistochemistry of kidney pathology on the seventh day after RUUO in model group and on the 1st, 3rd, 7th, and 14th day after RUUO in observation group. Results: Comparing with mice in RUUO group, mice in improved RUUO group had lower renal volume, tubular damage score, and collagen area percentage. After the obstruction was relieved, the renal volume decreased gradually within 2 weeks. The tubular damage score in 7d RUUO group was lower than that in 1d RUUO and 3d RUUO group. However, the tubular damage score in 14d RUUO group was higher than that in 7d RUUO group. The tendency of collagen area percentage and α-SMA IOD value were consistent with the tubular damage score. Conclusions: Using the method of reimplantation + catheter, a reliable mice model of RUUO can be got. After RUUO, the de-obstructed kidneys are still in damage and fibrosis state.
- Subjects :
- Male
medicine.medical_specialty
030232 urology & nephrology
030204 cardiovascular system & hematology
Urinary Catheters
Critical Care and Intensive Care Medicine
urologic and male genital diseases
Kidney
03 medical and health sciences
Mice
0302 clinical medicine
Laboratory Study
Renal fibrosis
Relief of unilateral ureteral obstruction
Medicine
Animals
Humans
Pathological
urogenital system
business.industry
General Medicine
catheter
renal fibrosis
Fibrosis
Diseases of the genitourinary system. Urology
Surgery
Mice, Inbred C57BL
Catheter
Disease Models, Animal
Nephrology
pathology
RC870-923
Ureter
business
Urinary Catheterization
Ureteral Obstruction
Subjects
Details
- Language :
- English
- ISSN :
- 15256049 and 0886022X
- Volume :
- 41
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Renal Failure
- Accession number :
- edsair.doi.dedup.....c00bb23d429903d99f4f953208c71af3