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Ethanol-induced apoptosis in vitro
Ethanol-induced apoptosis in vitro
- Publication Year :
- 1999
-
Abstract
- OBJECTIVES: The aim is to study the apoptotic process in a human hepatocyte model for ethanol (EtOH)-induced apoptosis. DESIGN AND METHODS: Normal human primary hepatocytes (HPH) and Hep G2 cells were exposed to increasing EtOH. 6000 cells/ sample were analyzed by transmission electron microscopy. RESULTS: Apoptotic cells were observed (mmol/L EtOH): 40: 6 +/-0.5%, 60:13 +/- 2% (p < 0.05), 80: 26 +/- 1% (p < 0.001) (vs. control). Two consecutive doses of 80 mmol/L for 24 h each additionally increased apoptosis 55 +/- 3% (p < 0.0001 vs. control and p < 0.001 vs. single dose). In response to this exposure, there is a stronger apoptotic activity in HPH when compared to Hep G2 (p < 0.05). CONCLUSIONS: In vitro, EtOH-induced apoptosis is regulated by dose level and the frequency of exposure.
- Subjects :
- endocrine system
Cells
Clinical Biochemistry
Liver/cytology
Apoptosis
Apoptosis/drug effects
Biology
Dose level
Electron
Liver/drug effects
Apoptosis/drug effects*, Cells, Cultured, Ethanol/pharmacology*, Humans, Liver/cytology, Liver/drug effects*, Liver/ultrastructure, Microscopy, Electron
chemistry.chemical_compound
Apoptotic Process
mental disorders
Mole
Humans
reproductive and urinary physiology
Cells, Cultured
Microscopy
Cultured
Ethanol
General Medicine
Liver/ultrastructure
Molecular biology
In vitro
Hep G2
Human hepatocyte
Microscopy, Electron
Biochemistry
chemistry
Liver
Ethanol/pharmacology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....c0179fbba79f17ea6f5ec01748957b2d