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Mir-30d suppresses cell proliferation of colon cancer cells by inhibiting cell autophagy and promoting cell apoptosis
- Source :
- Tumor Biology, Vol 39 (2017)
- Publication Year :
- 2017
-
Abstract
- MiR-30 family plays an important role in the tumorigenesis of human cancers. The aim of the study is to investigate the role of miR-30d in human colon cancer cell lines and explore the molecular mechanism in the proliferation of colon cancer cells. The expression of miR-30d was determined by real-time polymerase chain reaction assay in colon cancer cell lines (HCT15, HCT116, HT-29, DLD-1, and SW480) and the results demonstrated that miR-30d level was significantly decreased in human colon cancer cell lines, compared with normal colon epithelial cell line. Transfection with miR-30d mimics inhibited cell proliferation, and transfection with miR-30d inhibitors significantly promoted cell viability of colon cancer cells. Furthermore, TargetScan analysis predicted that miR-30d interacted with messenger RNA on its 3' untranslated region of ATG5, phosphoinositide 3-kinase, and Beclin1 to negatively regulate cell autophagy in colon cancer cells. Moreover, transfection with miR-30d induced cell arrest at G2/M phase of HT-29 cells. Overexpression of miR-30d mimics inhibited cell viability probably due to the inhibition of cell autophagy and promotion of cell apoptosis. Thus, MiR-30d inhibited cell autophagy by directly targeting messenger RNA of ATG5, phosphoinositide 3-kinase, and Beclin1 and promoted cell apoptosis of human colon cancer cells. It is helpful to clarify the function of miR-30d in tumorigenesis of human cancers.
- Subjects :
- 0301 basic medicine
Carcinogenesis
ATG5
Cell
Apoptosis
medicine.disease_cause
Transfection
Autophagy-Related Protein 5
03 medical and health sciences
Phosphatidylinositol 3-Kinases
0302 clinical medicine
medicine
Autophagy
Humans
Viability assay
RC254-282
Cell Proliferation
Cell growth
Chemistry
Neoplasms. Tumors. Oncology. Including cancer and carcinogens
General Medicine
Gene Expression Regulation, Neoplastic
MicroRNAs
030104 developmental biology
medicine.anatomical_structure
Cell culture
030220 oncology & carcinogenesis
Colonic Neoplasms
Cancer research
Beclin-1
HT29 Cells
Subjects
Details
- ISSN :
- 14230380
- Volume :
- 39
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine
- Accession number :
- edsair.doi.dedup.....c019df23c7a5f82370448b9d8157cac5