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Bacillus subtilis Glutamine Synthetase Controls Gene Expression through a Protein-Protein Interaction with Transcription Factor TnrA

Authors :
Jill M. Zalieckas
Susan H. Fisher
Lewis V. Wray
Source :
Cell. 107(4):427-435
Publication Year :
2001
Publisher :
Elsevier BV, 2001.

Abstract

Bacillus subtilis TnrA, a global regulator of transcription, responds to nitrogen availability, but the specific signal to which it responds has been elusive. Genetic studies indicate that glutamine synthetase is required for the regulation of TnrA activity in vivo. We report here that the feedback-inhibited form of glutamine synthetase directly interacts with TnrA and blocks the DNA binding activity of TnrA. Mutations in the tnrA gene ( tnrA C ) that allow constitutive high level expression of tnrA -activated genes were isolated and characterized. Feedback-inhibited glutamine synthetase had a significantly reduced ability to block the in vitro DNA binding by three of the TnrA C proteins. Thus, glutamine synthetase, an enzyme of central metabolism, directly interacts with and regulates the DNA binding activity of TnrA.

Details

ISSN :
00928674
Volume :
107
Issue :
4
Database :
OpenAIRE
Journal :
Cell
Accession number :
edsair.doi.dedup.....c01c31086937bf59e8aa380ebb0fa33a
Full Text :
https://doi.org/10.1016/s0092-8674(01)00572-4