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Testing if Micro-CT Is Capable of Quantitating the Extent of Proteoglycan-Aggrecan Induced Axial Spondyloarthritis in Mice
- Source :
- Frontiers in Immunology, Vol 12 (2021), Frontiers in Immunology
- Publication Year :
- 2021
- Publisher :
- Frontiers Media S.A., 2021.
-
Abstract
- ObjectiveInjections of proteoglycan aggrecan (PGA) have been reported to induce axial spondyloarthritis (ax-SpA) in BALB/c mice. It is considered to be a model for radiographic ax-SpA. However, evaluation of the extent of axial disease by histopathological assessment of every intervertebral space is labor-intensive. The objective of our paper is to test the feasibility of Micro Computed Tomography (Micro-CT) in rapidly enumerating the number of intervertebral spaces affected in each mouse.MethodsArthritis was induced in BALB/c mice by intraperitoneal injections of PGA. Involvement of several spinal segments, and selected sacroiliac and hip joints were evaluated by histopathology. The involvement of all intervertebral spaces, sacroiliac and hip joints was evaluated by Micro-CT.ResultsBALB/c mice injected with PGA developed histopathology of SpA-like axial lesions, including spondylitis, sacroiliac joint arthritis and hip joint arthritis. Micro-CT allowed us to clearly enumerate the number of lesions in each mouse.ConclusionMicro-CT allows quantitative assessment of the extent of axial involvement in PGA-induced mouse spondylitis. This can be a useful tool in assessing therapeutic interventions.
- Subjects :
- 0301 basic medicine
musculoskeletal diseases
Pathology
medicine.medical_specialty
Radiography
Immunology
Arthritis
BALB/c
03 medical and health sciences
Mice
0302 clinical medicine
Spondylarthritis
medicine
Immunology and Allergy
Animals
Aggrecans
Axial spondyloarthritis
Spondylitis
Aggrecan
Original Research
030203 arthritis & rheumatology
proteoglycan
biology
business.industry
X-Ray Microtomography
spondyloarthritis
RC581-607
medicine.disease
biology.organism_classification
Immunohistochemistry
animal models
Disease Models, Animal
030104 developmental biology
Proteoglycan
biology.protein
Cytokines
Histopathology
Female
Proteoglycans
aggrecan
Immunologic diseases. Allergy
business
Biomarkers
Subjects
Details
- Language :
- English
- ISSN :
- 16643224
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Frontiers in Immunology
- Accession number :
- edsair.doi.dedup.....c021d16db85f7a80c48a7f69cdff1315