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Tight modulation of Escherichia coli bacterial biofilm formation through controlled expression of adhesion factors
- Source :
- Applied and Environmental Microbiology, Applied and Environmental Microbiology, American Society for Microbiology, 2007, 73 (10), pp.3391-403. ⟨10.1128/AEM.02625-06⟩, Applied and Environmental Microbiology, 2007, 73 (10), pp.3391-403. ⟨10.1128/AEM.02625-06⟩
- Publication Year :
- 2007
- Publisher :
- HAL CCSD, 2007.
-
Abstract
- Despite the economic and sanitary problems caused by harmful biofilms, biofilms are nonetheless used empirically in industrial environmental and bioremediation processes and may be of potential use in medical settings for interfering with pathogen development. Escherichia coli is one of the bacteria with which biofilm formation has been studied in great detail, and it is especially appreciated for biotechnology applications because of its genetic amenability. Here we describe the development of two new genetic tools enabling the constitutive and inducible expression of any gene or operon of interest at its native locus. In addition to providing valuable tools for complementation and overexpression experiments, these two compact genetic cassettes were used to modulate the biofilm formation capacities of E. coli by taking control of two biofilm-promoting factors, autotransported antigen 43 adhesin and the bscABZC cellulose operon. The modulation of the biofilm formation capacities of E. coli or those of other bacteria capable of being genetically manipulated may be of use both for reducing and for improving the impact of biofilms in a number of industrial and medical applications.
- Subjects :
- Operon
MESH: Escherichia coli Proteins
MESH: Adhesins, Escherichia coli
medicine.disease_cause
Applied Microbiology and Biotechnology
Bacterial Adhesion
Genes, Reporter
Promoter Regions, Genetic
Recombination, Genetic
0303 health sciences
Adhesins, Escherichia coli
MESH: Gene Expression Regulation, Bacterial
Ecology
biology
MESH: Escherichia coli
Escherichia coli Proteins
Physiology and Biotechnology
Enterobacteriaceae
Complementation
MESH: beta-Galactosidase
MESH: Promoter Regions (Genetics)
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
MESH: Recombination, Genetic
Biotechnology
MESH: Operon
Green Fluorescent Proteins
MESH: Biofilms
Microbiology
03 medical and health sciences
MESH: Green Fluorescent Proteins
Cellulase
medicine
Escherichia coli
MESH: Bacterial Adhesion
Gene
030304 developmental biology
030306 microbiology
MESH: Genes, Reporter
Biofilm
MESH: Cellulase
Gene Expression Regulation, Bacterial
biochemical phenomena, metabolism, and nutrition
biology.organism_classification
beta-Galactosidase
Bacterial adhesin
Biofilms
Bacteria
Food Science
Subjects
Details
- Language :
- English
- ISSN :
- 00992240 and 10985336
- Database :
- OpenAIRE
- Journal :
- Applied and Environmental Microbiology, Applied and Environmental Microbiology, American Society for Microbiology, 2007, 73 (10), pp.3391-403. ⟨10.1128/AEM.02625-06⟩, Applied and Environmental Microbiology, 2007, 73 (10), pp.3391-403. ⟨10.1128/AEM.02625-06⟩
- Accession number :
- edsair.doi.dedup.....c02fed8dd0beb0f188306f86838fe29c