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CRA(Crosolic Acid) isolated from Actinidia valvata Dunn.Radix induces apoptosis of human gastric cancer cell line BGC823 in vitro via down-regulation of the NF-κB pathway
- Source :
- Food and Chemical Toxicology. 105:475-485
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- A natural ursolic compound, 2α,3β-dihydroxy-urs-12-en-28-oic acid (corosolic acid, CRA) was isolated from the root of Actinidia valvata Dunn. (A. valvata Radix). Since a large number of triterpenoid compound has marked anticancer effects toward various types of cancer cell lines in vitro, this study was carried out to investigate the anticancer effect of CRA in human gastric cancer cell line BGC823 cells and the underlying apoptotic mechanism of CRA was examined in BGC823 cell lines. The results showed that CRA significantly suppressed the viability of BGC823 cells in a concentration- and time-dependent manner. CRA also significantly increased the sub G1 population by cell cycle analysis in a concentration dependent manner. Exposure to CRA decreased p65, bcl-2, Fas, smac mRNA and protein expression, and increased IκBα, bax, survivin mRNA and protein expression. Results of immunofluorescence staining and EMSA further indicated CRA induced apoptosis by inhibiting nuclear translocation of nuclear factor NF-κB subunit p65. Consistently overall, our findings suggest that CRA induces apoptosis via inhibition of NF-κB (p65) expression level and activation of IκBα in BGC cells as a potent anticancer candidate for gastric cancer treatment.
- Subjects :
- 0301 basic medicine
genetic structures
Actinidia
Population
Down-Regulation
Apoptosis
Biology
Toxicology
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Stomach Neoplasms
Cell Line, Tumor
Survivin
Humans
education
Cell Proliferation
education.field_of_study
Plant Extracts
NF-kappa B
NF-κB
General Medicine
Molecular biology
Triterpenes
In vitro
IκBα
030104 developmental biology
chemistry
Biochemistry
Cell culture
030220 oncology & carcinogenesis
Corosolic acid
Signal Transduction
Food Science
Subjects
Details
- ISSN :
- 02786915
- Volume :
- 105
- Database :
- OpenAIRE
- Journal :
- Food and Chemical Toxicology
- Accession number :
- edsair.doi.dedup.....c03425a6fbd84284bdf8d7e8c7acbe55