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LncRNA TUG1 sponges microRNA-9 to promote neurons apoptosis by up-regulated Bcl2l11 under ischemia
- Source :
- Biochemical and biophysical research communications. 485(1)
- Publication Year :
- 2017
-
Abstract
- Emerging studies have illustrated that LncRNAs TUG1 play critical roles in multiple biologic processes. However, the LncRNA TUG1 expression and function in ischemic stroke have not been reported yet. In this study, we found that LncRNA TUG1 expression was significantly up-regulated in brain ischemic penumbra from rat middle carotid artery occlusion (MCAO) model, while similar results were also observed in cultured neurons under oxygen-glucose deprivation (OGD) insult. Knockdown of TUG1 decreased the ratio of apoptotic cells and promoted cells survival in vitro, which may be regulated by the elevated miRNA-9 expression and decreased Bcl2l11 protein. Furthermore, TUG1 could directly interact with miR-9 and down-regulating miR-9 could efficiently reverse the function of TUG1 on the Bcl2l11 expression. In summary, our result sheds light on the role of LncRNA TUG1 as a miRNA sponge for ischemic stroke, possibly providing a new therapeutic target in stroke.
- Subjects :
- 0301 basic medicine
Male
Biophysics
Ischemia
Apoptosis
Biology
Bioinformatics
Biochemistry
Brain Ischemia
Rats, Sprague-Dawley
03 medical and health sciences
0302 clinical medicine
Downregulation and upregulation
microRNA
medicine
Animals
Molecular Biology
Stroke
Cells, Cultured
Neurons
Gene knockdown
Bcl-2-Like Protein 11
Penumbra
Brain
Cell Biology
medicine.disease
Cell biology
Up-Regulation
MicroRNAs
030104 developmental biology
BCL2L11
Gene Expression Regulation
Gene Knockdown Techniques
RNA, Long Noncoding
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 10902104
- Volume :
- 485
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Biochemical and biophysical research communications
- Accession number :
- edsair.doi.dedup.....c05a0ee1eb31ce1cd29792b92523e052