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Mammalian nucleolar protein DCAF13 is essential for ovarian follicle maintenance and oocyte growth by mediating rRNA processing
- Source :
- Cell Death Differ
- Publication Year :
- 2018
- Publisher :
- Springer Science and Business Media LLC, 2018.
-
Abstract
- During mammalian oocyte growth, chromatin configuration transition from the nonsurrounded nucleolus (NSN) to surrounded nucleolus (SN) type plays a key role in the regulation of gene expression and acquisition of meiotic and developmental competence by the oocyte. Nonetheless, the mechanism underlying chromatin configuration maturation in oocytes is poorly understood. Here we show that nucleolar protein DCAF13 is an important component of the ribosomal RNA (rRNA)-processing complex and is essential for oocyte NSN-SN transition in mice. A conditional knockout of Dcaf13 in oocytes led to the arrest of oocyte development in the NSN configuration, follicular atresia, premature ovarian failure, and female sterility. The DCAF13 deficiency resulted in pre-rRNA accumulation in oocytes, whereas the total mRNA level was not altered. Further exploration showed that DCAF13 participated in the 18S rRNA processing in growing oocytes. The lack of 18S rRNA because of DCAF13 deletion caused a ribosome assembly disorder and then reduced global protein synthesis. DCAF13 interacted with a protein of the core box C/D ribonucleoprotein, fibrillarin, i.e., a factor of early pre-rRNA processing. When fibrillarin was knocked down in the oocytes from primary follicles, follicle development was inhibited as well, indicating that an rRNA processing defect in the oocyte indeed stunts chromatin configuration transition and follicle development. Taken together, these results elucidated the in vivo function of novel nucleolar protein DCAF13 in maintaining mammalian oogenesis.
- Subjects :
- 0301 basic medicine
Nucleolus
Biology
Oogenesis
Article
Ribosome assembly
Mice
03 medical and health sciences
0302 clinical medicine
Ovarian Follicle
medicine
Animals
Humans
RNA Processing, Post-Transcriptional
RRNA processing
Molecular Biology
Cells, Cultured
Fibrillarin
Follicular atresia
RNA-Binding Proteins
Cell Biology
Oocyte
Chromatin
Cell biology
Mice, Inbred C57BL
030104 developmental biology
medicine.anatomical_structure
RNA, Ribosomal
030220 oncology & carcinogenesis
Oocytes
Female
HeLa Cells
Subjects
Details
- ISSN :
- 14765403 and 13509047
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Cell Death & Differentiation
- Accession number :
- edsair.doi.dedup.....c0864c77baa183dde77d770c972909f4