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The Mitochondrial Permeability Transition Regulates Cytochrome c Release for Apoptosis during Endoplasmic Reticulum Stress by Remodeling the Cristae Junction
- Source :
- Journal of Biological Chemistry. 283:3476-3486
- Publication Year :
- 2008
- Publisher :
- Elsevier BV, 2008.
-
Abstract
- The role of the mitochondrial permeability transition (MPT) in apoptosis and necrosis is controversial. Here we show that the MPT regulates the release of cytochrome c for apoptosis during endoplasmic reticulum (ER) stress by remodeling the cristae junction (CJ). CEM cells, HCT116 colon cancer cells, and murine embryo fibroblast cells were treated with the ER stressor thapsigargin (THG), which led to cyclophilin D-dependent mitochondrial release of the profusion GTPase optic atrophy 1 (OPA1), which controls CJ integrity, and cytochrome c, leading to apoptosis. Interference RNA knockdown of Bax blocked OPA1 and cytochrome c release after THG treatment but did not prevent the MPT, showing that Bax was essential for the release of cytochrome c by MPT. In isolated mitochondria, MPT led to OPA1 and cytochrome c release independently of voltage-dependent anion channel and the outer membrane, indicating that the MPT is an inner membrane phenomenon. Last, the MPT was regulated by the electron transport chain but not mitochondrial reactive oxygen species, since THG-induced cell death was not blocked by antioxidants and did not occur in cells lacking mitochondrial DNA. Our results show that the MPT regulates CJ remodeling for cytochrome c-dependent apoptosis induced by ER stress and that mitochondrial electron transport is indispensable for this process.
- Subjects :
- Cytochrome
Apoptosis
Mice, Transgenic
Endoplasmic Reticulum
Biochemistry
Mitochondrial apoptosis-induced channel
Permeability
GTP Phosphohydrolases
Membrane Potentials
Mice
Cell Line, Tumor
medicine
Animals
Humans
Molecular Biology
biology
Endoplasmic reticulum
Cytochrome c
Cytochromes c
Cell Biology
medicine.disease
Molecular biology
Mitochondria
Cell biology
Gene Expression Regulation
Mitochondrial permeability transition pore
biology.protein
Optic Atrophy 1
Apoptosome
Reactive Oxygen Species
Subjects
Details
- ISSN :
- 00219258
- Volume :
- 283
- Database :
- OpenAIRE
- Journal :
- Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....c096ce71d60104b809277d975f837157