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Evolutionary conservation of the folding nucleus

Authors :
Mirny, Leonid
Shakhnovich, Eugene
Publication Year :
2000
Publisher :
arXiv, 2000.

Abstract

In this Communication we present statistical analysis of conservation profiles in families of homologous sequences for nine proteins whose folding nucleus was determined by protein engineering methods. We show that in all but one protein (AcP) folding nucleus residues are significantly more conserved than the rest of the protein. Two aspects of our study are especially important: 1) grouping of amino acids into classes according to their physical-chemical properties and 2) proper normalization of amino acid probabilities that reflects the fact that evolutionary pressure to conserve some amino acid types may itself affect concentration of various amino acid types in protein families. Neglect of any of those two factors may make physical and biological ``signals'' from conservation profiles disappear.<br />Comment: submitted to Journal of Molecular Biology

Details

Database :
OpenAIRE
Accession number :
edsair.doi.dedup.....c0d07d1348fef2b3b2c15ff96be949b3
Full Text :
https://doi.org/10.48550/arxiv.physics/0012003