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Usefulness of Dura Mater in Providing DNA Samples for Identifying Cadavers
- Source :
- Journal of Forensic Sciences. 51:888-892
- Publication Year :
- 2006
- Publisher :
- Wiley, 2006.
-
Abstract
- We examined the usefulness of the dura mater in identifying human remains. Dura mater was collected from 50 cadavers, including drowned, charred, and mummified remains. The STR genotype using the AmpFlSTR® Identifilerâ„¢ Kit could be typed at 15 STR and amelogenin loci in 30 samples of 33 cases. Furthermore, the ABO genotype and amelogenin using gel-based methods could be typed in 44 samples of 50 cases. In cases with successful typing of STR, ABO-DNA, and amelogenin, the longest time after death was from 12 to 26 days in a drowned body. The minimum quantity of dura mater required for DNA extraction was about 2.5 mg, dried and fixed by ethanol, in a cadaver 15 h after death. The state of the DNA from the dura mater from the calvaria may be better than that from the basis cranii interna. We found that DNA from dura mater is one of the most useful samples for forensic identification.
- Subjects :
- Adult
Male
Pathology
medicine.medical_specialty
Adolescent
Genotype
Dura mater
Biology
Polymerase Chain Reaction
ABO Blood-Group System
Pathology and Forensic Medicine
Dental Enamel Proteins
Cadaver
Genetics
medicine
Humans
Basis cranii interna
Child
Aged
Aged, 80 and over
Amelogenin
Infant
DNA
Anatomy
Middle Aged
Sex Determination Processes
DNA Fingerprinting
DNA extraction
Forensic identification
medicine.anatomical_structure
Tandem Repeat Sequences
Child, Preschool
Abo genotype
Forensic Anthropology
Female
Dura Mater
Subjects
Details
- ISSN :
- 15564029 and 00221198
- Volume :
- 51
- Database :
- OpenAIRE
- Journal :
- Journal of Forensic Sciences
- Accession number :
- edsair.doi.dedup.....c0dfaf4fd7ff847b39bdd2c902b836c0