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The giemsa banding pattern of canine chromosomes, using a cell synchronization technique
- Source :
- Genome. 34(3)
- Publication Year :
- 1991
-
Abstract
- Cytogenetic investigations of the domestic dog, Canis familiaris, were performed on one Doberman pinscher and two Boxer dogs. Conventional homogeneously stained and G-banded metaphases from peripheral blood lymphocyte cultures synchronized with amethopterin and bromodeoxyuridine were studied. These procedures permitted the unequivocal identification of all canine chromosomes. A canine chromosome idiogram was constructed on the basis of the G-banding pattern at the haploid 327-band resolution level. The secondary constrictions and tapering of the telomeric regions characteristic of several canine chromosomes are described. Q-, C-, and NOR-banding were also performed and the salient features are described. This karyotype should enhance the value of the canine species in cytogenetic investigations.Key words: canine karyotype, G-banding, Q-banding, C-banding, NOR-banding, cell synchronization idiogram.
- Subjects :
- Male
medicine.medical_specialty
X Chromosome
G banding
Biology
Azure Stains
Amethopterin
Cytogenetics
Dogs
Genetics
medicine
Animals
Molecular Biology
Cells, Cultured
Metaphase
Chromosome
Karyotype
General Medicine
Anatomy
Molecular biology
Doberman Pinscher
Chromosome Banding
Karyotyping
Female
Nucleolus organizer region
Ploidy
Biotechnology
Subjects
Details
- ISSN :
- 08312796
- Volume :
- 34
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Genome
- Accession number :
- edsair.doi.dedup.....c13e46b9eac0afd6db57ef56405f77fc