Hydrogen deuterium mass spectrometry in drug discovery

Hydrogen deuterium exchange mass spectrometry (HDX-MS) has become an increasingly routine technology for probing the solution structure and dynamics of proteins. HDX-MS measures the exchange of deuterium with the amide proton on the protein backbone chain. HDX-MS results can be used to aid in construct design for biophysical studies, to probe protein–ligand interactions and to characterize the dynamics of proteins. This digest highlights recent progress which makes this technology accessible and explores examples of HDX-MS in drug discovery and solution state structural biology.