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Intracellular fate of carbon nanotubes inside murine macrophages: pH-dependent detachment of iron catalyst nanoparticles
- Source :
- Particle and Fibre Toxicology, Particle and Fibre Toxicology, BioMed Central, 2013, 10 (1), pp.24. ⟨10.1186/1743-8977-10-24⟩, Particle and Fibre Toxicology, 2013, 10 (1), pp.24. ⟨10.1186/1743-8977-10-24⟩, 'Particle and Fibre Toxicology ', vol: 10, pages: 24-1-24-12 (2013), Bussy, C, Paineau, E, Cambedouzou, J, Brun, N, Mory, C, Fayard, B, Salomé, M, Pinault, M, Huard, M, Belade, E, Armand, L, Boczkowski, J, Launois, P & Lanone, S 2013, ' Intracellular fate of carbon nanotubes inside murine macrophages: PH-dependent detachment of iron catalyst nanoparticles ', Particle and Fibre Toxicology, vol. 10, no. 1, 24 . https://doi.org/10.1186/1743-8977-10-24
- Publication Year :
- 2013
- Publisher :
- HAL CCSD, 2013.
-
Abstract
- Background Carbon nanotubes (CNT) are a family of materials featuring a large range of length, diameter, numbers of walls and, quite often metallic impurities coming from the catalyst used for their synthesis. They exhibit unique physical properties, which have already led to an extensive development of CNT for numerous applications. Because of this development and the resulting potential increase of human exposure, an important body of literature has been published with the aim to evaluate the health impact of CNT. However, despite evidences of uptake and long-term persistence of CNT within macrophages and the central role of those cells in the CNT-induced pulmonary inflammatory response, a limited amount of data is available so far on the CNT fate inside macrophages. Therefore, the overall aim of our study was to investigate the fate of pristine single walled CNT (SWCNT) after their internalization by macrophages. Methods To achieve our aim, we used a broad range of techniques that aimed at getting a comprehensive characterization of the SWCNT and their catalyst residues before and after exposure of murine macrophages: X-ray diffraction (XRD), High Resolution (HR) Transmission Electron Microscopy (TEM), High Angle Annular Dark Field-Scanning TEM (HAADF-STEM) coupled to Electron Energy Loss Spectroscopy (EELS), as well as micro-X-ray fluorescence mapping (μXRF), using synchrotron radiation. Results We showed 1) the rapid detachment of part of the iron nanoparticles initially attached to SWCNT which appeared as free iron nanoparticles in the cytoplasm and nucleus of CNT-exposed murine macrophages, and 2) that blockade of intracellular lysosomal acidification prevented iron nanoparticles detachment from CNT bundles and protected cells from CNT downstream toxicity. Conclusions The present results, while obtained with pristine SWCNT, could likely be extended to other catalyst-containing nanomaterials and surely open new ways in the interpretation and understanding of CNT toxicity.
- Subjects :
- Materials science
Health, Toxicology and Mutagenesis
Carbon nanotubes
Metal Nanoparticles
Nanoparticle
Nanotechnology
Spectroscopy, Electron Energy-Loss
02 engineering and technology
Carbon nanotube
Toxicology
Cathepsin B
Cell Line
law.invention
Nanomaterials
Catalysis
Mice
03 medical and health sciences
Degradation
Microscopy, Electron, Transmission
X-Ray Diffraction
law
Animals
030304 developmental biology
0303 health sciences
Toxicity
Nanotubes, Carbon
Research
Macrophages
Electron energy loss spectroscopy
Lysosome-Associated Membrane Glycoproteins
Spectrometry, X-Ray Emission
General Medicine
Hydrogen-Ion Concentration
021001 nanoscience & nanotechnology
Fluorescence
Lysosome acidification
Iron catalyst nanoparticles
Transmission electron microscopy
[SDV.SPEE] Life Sciences [q-bio]/Santé publique et épidémiologie
Biophysics
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
Macrolides
Lysosomes
0210 nano-technology
Iron Compounds
Synchrotrons
Intracellular
Subjects
Details
- Language :
- English
- ISSN :
- 17438977
- Database :
- OpenAIRE
- Journal :
- Particle and Fibre Toxicology, Particle and Fibre Toxicology, BioMed Central, 2013, 10 (1), pp.24. ⟨10.1186/1743-8977-10-24⟩, Particle and Fibre Toxicology, 2013, 10 (1), pp.24. ⟨10.1186/1743-8977-10-24⟩, 'Particle and Fibre Toxicology ', vol: 10, pages: 24-1-24-12 (2013), Bussy, C, Paineau, E, Cambedouzou, J, Brun, N, Mory, C, Fayard, B, Salomé, M, Pinault, M, Huard, M, Belade, E, Armand, L, Boczkowski, J, Launois, P & Lanone, S 2013, ' Intracellular fate of carbon nanotubes inside murine macrophages: PH-dependent detachment of iron catalyst nanoparticles ', Particle and Fibre Toxicology, vol. 10, no. 1, 24 . https://doi.org/10.1186/1743-8977-10-24
- Accession number :
- edsair.doi.dedup.....c14cc7cff61de82e30c9d265a3a2f2c7