Aberrations of Chromosomes 1 and 17 in Six Human Osteosarcoma Cell Lines Using Double-Target Fluorescence In Situ Hybridization

Analysis of six human osteosarcoma cell lines was performed by using double-target fluorescence in situ hybridization (FISH). FISH was applied to interphase nuclei, not to metaphase chromosomes. In this study, numerical aberrations of chromosomes 1 and 17 or structural chromosomal aberrations of chromosome arm 1p or 17p, in which it has been suggested that there are one or more tumor suppressor genes in various malignant tumors, were examined with this technique. All six of the human osteosarcoma cell lines studied had extra copies of chromosomes 1 and 17. A high frequency of deletions (>60%) in chromosome 1 was found in two cell lines and deletions of chromosome 17 were found in one cell line.