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Transient coating of γ-Fe2O3 nanoparticles with glutamate for its delivery to and removal from brain nerve terminals
- Source :
- Beilstein Journal of Nanotechnology, Beilstein Journal of Nanotechnology, Vol 11, Iss 1, Pp 1381-1393 (2020)
- Publication Year :
- 2020
- Publisher :
- Beilstein-Institut, 2020.
-
Abstract
- Glutamate is the main excitatory neurotransmitter in the central nervous system and excessive extracellular glutamate concentration is a characteristic feature of stroke, brain trauma, and epilepsy. Also, glutamate is a potential tumor growth factor. Using radiolabeled ʟ-[14C]glutamate and magnetic fields, we developed an approach for monitoring the biomolecular coating (biocoating) with glutamate of the surface of maghemite (γ-Fe2O3) nanoparticles. The nanoparticles decreased the initial rate of ʟ-[14C]glutamate uptake, and increased the ambient level of ʟ-[14C]glutamate in isolated cortex nerve terminals (synaptosomes). The nanoparticles exhibit a high capability to adsorb glutamate/ʟ-[14C]glutamate in water. Some components of the incubation medium of nerve terminals, that is, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and NaH2PO4, decreased the ability of γ-Fe2O3 nanoparticles to form a glutamate biocoating by about 50% and 90%, respectively. Only 15% of the amount of glutamate biocoating obtained in water was obtained in blood plasma. Albumin did not prevent the formation of a glutamate biocoating. It was shown that the glutamate biocoating is a temporal dynamic structure at the surface of γ-Fe2O3 nanoparticles. Also, components of the nerve terminal incubation medium and physiological fluids responsible for the desorption of glutamate were identified. Glutamate-coated γ-Fe2O3 nanoparticles can be used for glutamate delivery to the nervous system or for glutamate adsorption (but with lower effectiveness) in stroke, brain trauma, epilepsy, and cancer treatment following by its subsequent removal using a magnetic field. γ-Fe2O3 nanoparticles with transient glutamate biocoating can be useful for multifunctional theranostics.
- Subjects :
- Nervous system
Central nervous system
General Physics and Astronomy
Nanoparticle
02 engineering and technology
lcsh:Chemical technology
lcsh:Technology
Full Research Paper
03 medical and health sciences
chemistry.chemical_compound
Blood plasma
medicine
Nanotechnology
lcsh:TP1-1185
General Materials Science
Electrical and Electronic Engineering
lcsh:Science
030304 developmental biology
HEPES
0303 health sciences
protein biocorona
lcsh:T
Chemistry
Glutamate receptor
Albumin
brain nerve terminals
maghemite (γ-Fe2O3) nanoparticles
021001 nanoscience & nanotechnology
lcsh:QC1-999
Cortex (botany)
Nanoscience
medicine.anatomical_structure
glutamate biocoating
Biophysics
lcsh:Q
0210 nano-technology
lcsh:Physics
blood plasma
Subjects
Details
- Language :
- English
- ISSN :
- 21904286
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- Beilstein Journal of Nanotechnology
- Accession number :
- edsair.doi.dedup.....c17e83077ce99722a892d3cb22259e03