Back to Search
Start Over
Loss of PINK1 in medaka fish (Oryzias latipes) causes late-onset decrease in spontaneous movement
- Source :
- Neuroscience Research. 66:151-161
- Publication Year :
- 2010
- Publisher :
- Elsevier BV, 2010.
-
Abstract
- Parkinson's disease is a neurodegenerative disease associated with the degeneration of dopaminergic neurons in the substantia nigra. The PTEN-induced kinase 1 gene (PINK1) is responsible for recessive inherited familial Parkinson's disease (PARK6). Neither the function of PINK1 nor its role in the prevention of Parkinson's disease is fully understood. Gene disruption of PINK1 causes remarkably different phenotypes in animal models such as Drosophila melanogaster, zebrafish, and mouse, none of which recapitulate Parkinson's-disease-like symptoms. We established PINK1-gene-disrupted medaka fish. These mutant fish grew normally at first, then developed significant decrease in the frequency of spontaneous swimming movements in the late-adult stage. Although the mutants did not show any dopaminergic cell loss, the amount of 3,4-dihydroxyphenylacetic acid, a major metabolite of dopamine, decreased. Thus, PINK1 contributes to the maintenance of dopamine metabolism, even before the selective death of dopaminergic neurons. Our animal model is therefore a valuable tool to detect pathogenesis in Parkinson's patients in the early stages.
- Subjects :
- Fish Proteins
medicine.medical_specialty
Parkinson's disease
Dopamine
Movement
Ubiquitin-Protein Ligases
Oryzias
Blotting, Western
PINK1
Substantia nigra
Animals, Genetically Modified
Microscopy, Electron, Transmission
Dopaminergic Cell
Internal medicine
medicine
Animals
RNA, Messenger
Zebrafish
Chromatography, High Pressure Liquid
In Situ Hybridization
Swimming
Neurons
Analysis of Variance
Behavior, Animal
Staining and Labeling
biology
Reverse Transcriptase Polymerase Chain Reaction
General Neuroscience
Body Weight
Dopaminergic
Age Factors
Brain
General Medicine
biology.organism_classification
medicine.disease
Immunohistochemistry
Endocrinology
Mutation
Protein Kinases
medicine.drug
Subjects
Details
- ISSN :
- 01680102
- Volume :
- 66
- Database :
- OpenAIRE
- Journal :
- Neuroscience Research
- Accession number :
- edsair.doi.dedup.....c184caa261be34d72131b218fd0a0b3e