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Modulating the activity of the channel-forming segment of Vpr protein from HIV-1
- Source :
- European biophysics journal : EBJ. 39(7)
- Publication Year :
- 2009
-
Abstract
- Viral protein of regulation (Vpr) encoded by human immunodeficiency virus type 1 (HIV-1) is a short auxiliary protein that is 96 amino acids in length. During the viral life cycle, Vpr is released into the blood serum and is able to enter cellular membranes of noninfected cells. In this study a short peptide, Vpr(55-83), was shown to exhibit ion-channel-like activity when reconstituted into (1) planar lipid bilayers and (2) lipid bilayers held at the tip of a glass pipette. The two set-ups led to differences in the oligomerization state of the peptide, which was reflected in differences in the conductance levels. Experiments under applied hydrostatic pressure affect the dynamics of the protein within the membrane.
- Subjects :
- Viral protein
viruses
Hydrostatic pressure
Lipid Bilayers
Biophysics
Peptide
Biology
medicine.disease_cause
Membrane Potentials
Blood serum
medicine
Pressure
Lipid bilayer
chemistry.chemical_classification
Phosphatidylethanolamines
Lipid bilayer fusion
General Medicine
vpr Gene Products, Human Immunodeficiency Virus
Amino acid
Membrane protein
chemistry
Biochemistry
HIV-1
Phosphatidylcholines
Peptides
Subjects
Details
- ISSN :
- 14321017
- Volume :
- 39
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- European biophysics journal : EBJ
- Accession number :
- edsair.doi.dedup.....c19489eae0d91d7d3df75ece9e362a3e