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Transcriptome of American Oysters, Crassostrea virginica, in Response to Bacterial Challenge: Insights into Potential Mechanisms of Disease Resistance
- Source :
- PLoS ONE, PLoS ONE, Vol 9, Iss 8, p e105097 (2014)
- Publication Year :
- 2014
- Publisher :
- Public Library of Science (PLoS), 2014.
-
Abstract
- The American oyster Crassostrea virginica, an ecologically and economically important estuarine organism, can suffer high mortalities in areas in the Northeast United States due to Roseovarius Oyster Disease (ROD), caused by the gram-negative bacterial pathogen Roseovarius crassostreae. The goals of this research were to provide insights into: 1) the responses of American oysters to R. crassostreae, and 2) potential mechanisms of resistance or susceptibility to ROD. The responses of oysters to bacterial challenge were characterized by exposing oysters from ROD-resistant and susceptible families to R. crassostreae, followed by high-throughput sequencing of cDNA samples from various timepoints after disease challenge. Sequence data was assembled into a reference transcriptome and analyzed through differential gene expression and functional enrichment to uncover genes and processes potentially involved in responses to ROD in the American oyster. While susceptible oysters experienced constant levels of mortality when challenged with R. crassostreae, resistant oysters showed levels of mortality similar to non-challenged oysters. Oysters exposed to R. crassostreae showed differential expression of transcripts involved in immune recognition, signaling, protease inhibition, detoxification, and apoptosis. Transcripts involved in metabolism were enriched in susceptible oysters, suggesting that bacterial infection places a large metabolic demand on these oysters. Transcripts differentially expressed in resistant oysters in response to infection included the immune modulators IL-17 and arginase, as well as several genes involved in extracellular matrix remodeling. The identification of potential genes and processes responsible for defense against R. crassostreae in the American oyster provides insights into potential mechanisms of disease resistance.
- Subjects :
- Oyster
lcsh:Medicine
Gene Expression
Aquaculture
Pathogenesis
Pathology and Laboratory Medicine
Bioinformatics
Biochemistry
Immune Receptors
Transcriptome
0302 clinical medicine
Invertebrate Genomics
Medicine and Health Sciences
Rhodobacteraceae
lcsh:Science
Pathogen
Animal Management
Innate Immune System
0303 health sciences
Immune System Proteins
Multidisciplinary
biology
Roseovarius
food and beverages
Agriculture
Infectious Disease Immunology
Genomics
030220 oncology & carcinogenesis
Host-Pathogen Interactions
Cytokines
Crassostrea
Transcriptome Analysis
geographic locations
Research Article
Next-Generation Sequencing
animal structures
Immunology
Plant disease resistance
Microbiology
03 medical and health sciences
Immune system
biology.animal
Genetics
Animals
14. Life underwater
Gene
030304 developmental biology
Interleukins
lcsh:R
fungi
Immunity
Biology and Life Sciences
Proteins
Computational Biology
Molecular Development
Genome Analysis
biology.organism_classification
Ostreidae
Gene Expression Regulation
Animal Genomics
Immune System
bacteria
lcsh:Q
Clinical Immunology
Pattern Recognition Receptors
Genome Expression Analysis
Animal Genetics
Developmental Biology
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....c1a6e149e12934b554f65f3337874dda
- Full Text :
- https://doi.org/10.1371/journal.pone.0105097