Back to Search
Start Over
In Situ Preconditioning of Human Mesenchymal Stem Cells Elicits Comprehensive Cardiac Repair Following Myocardial Infarction
- Source :
- International Journal of Molecular Sciences, International Journal of Molecular Sciences, Vol 22, Iss 1449, p 1449 (2021), Volume 22, Issue 3
- Publication Year :
- 2021
-
Abstract
- Human bone marrow-derived mesenchymal stem cells (BM-MSCs), represented as a population of adult stem cells, have long been considered as one of the most promising sources for cell-based cardiac regenerative therapy. However, their clinical use has been significantly hampered by low survival and poor retention following administration into failing hearts. Here, to improve the therapeutic effectiveness of BM-MSCs, we examined a novel therapeutic platform named in situ preconditioning in a rat myocardial infarction (MI) model. In situ preconditioning was induced by a combinatory treatment of BM-MSCs with genetically engineered hepatocyte growth factor-expressing MSCs (HGF-eMSCs) and heart-derived extracellular matrix (hdECM) hydrogel. Subsequently, our results demonstrated that in situ preconditioning with cell mixture substantially improved the survival/retention of BM-MSCs in the MI-induced rat hearts. Enhanced retention of BM-MSCs ultimately led to a significant cardiac function improvement, which was derived from the protection of myocardium and enhancement of vessel formation in the MI hearts. The results provide compelling evidence that in situ preconditioning devised to improve the therapeutic potential of BM-MSCs can be an effective strategy to achieve cardiac repair of MI hearts.
- Subjects :
- 0301 basic medicine
Male
Cell
Cell- and Tissue-Based Therapy
Myocardial Infarction
Myocardial Ischemia
030204 cardiovascular system & hematology
Regenerative medicine
lcsh:Chemistry
Mice
0302 clinical medicine
Medicine
Myocardial infarction
lcsh:QH301-705.5
Spectroscopy
education.field_of_study
Neovascularization, Pathologic
Hepatocyte Growth Factor
Stem Cells
General Medicine
Middle Aged
Coronary Vessels
Computer Science Applications
Extracellular Matrix
vascular regeneration
Platelet Endothelial Cell Adhesion Molecule-1
medicine.anatomical_structure
Treatment Outcome
Echocardiography
Hepatocyte growth factor
medicine.drug
Adult stem cell
Cardiac function curve
Adult
Population
Mice, Nude
Bone Marrow Cells
Mesenchymal Stem Cell Transplantation
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
Young Adult
human mesenchymal stem cells
Animals
Humans
Regeneration
Physical and Theoretical Chemistry
Cardiac Surgical Procedures
education
Molecular Biology
Tissue Engineering
business.industry
Myocardium
Organic Chemistry
Mesenchymal stem cell
Mesenchymal Stem Cells
engineered mesenchymal stem cells
medicine.disease
Fibrosis
Rats
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
Cancer research
business
Subjects
Details
- ISSN :
- 14220067
- Volume :
- 22
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- International journal of molecular sciences
- Accession number :
- edsair.doi.dedup.....c1a7e6edcc58b04cd57b96a008ad9400