Back to Search
Start Over
Characterization of Candida albicans antigenic determinants by two-dimensional polyacrylamide gel electrophoresis and enhanced chemiluminescence
- Source :
- FEMS immunology and medical microbiology. 23(4)
- Publication Year :
- 1999
-
Abstract
- The use of a two-dimensional polyacrylamide gel electrophoresis joined with Western blotting allowed us to investigate the reactivities of antibodies present in sera from mice and humans to antigens of Candida albicans blastoconidia. The analysis of the antibody response in the two models studied and the comparison between the antibody response in infected and non-infected individuals showed that the infection by C. albicans produces changes in the antibody response which may be of relevance in the serodiagnosis of invasive candidiasis. These changes include the induction of antibodies against new antigens, the disappearance of antibodies against a group of antigens and variations in the reactivity of antibodies directed to a different group of antigens. The technique used resolved the isoforms of several antigens including enolase. It is concluded that the antibody response in humans and mice with candidiasis is not homogeneously directed to all the isoforms of an antigen.
- Subjects :
- Microbiology (medical)
Antigens, Fungal
Immunology
Biology
Microbiology
Epitope
Epitopes
Mice
Western blot
Antigen
Candida albicans
medicine
Immunology and Allergy
Animals
Humans
Electrophoresis, Gel, Two-Dimensional
Polyacrylamide gel electrophoresis
Antibodies, Fungal
Antiserum
medicine.diagnostic_test
General Medicine
biology.organism_classification
Molecular biology
Corpus albicans
Infectious Diseases
Luminescent Measurements
biology.protein
Antibody
Subjects
Details
- ISSN :
- 09288244
- Volume :
- 23
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- FEMS immunology and medical microbiology
- Accession number :
- edsair.doi.dedup.....c1ae51c3de82e984988ad8b4069cd112