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Urinary Extracellular Vesicles: A Position Paper by the Urine Task Force of the International Society for Extracellular Vesicles
- Source :
- Erdbrügger, U, Blijdorp, C J, Bijnsdorp, I V, Borràs, F E, Burger, D, Bussolati, B, Byrd, J B, Clayton, A, Dear, J W, Falcón-Pérez, J M, Grange, C, Hill, A F, Holthöfer, H, Hoorn, E J, Jenster, G, Jimenez, C R, Junker, K, Klein, J, Knepper, M A, Koritzinsky, E H, Luther, J M, Lenassi, M, Leivo, J, Mertens, I, Musante, L, Oeyen, E, Puhka, M, van Royen, M E, Sánchez, C, Soekmadji, C, Thongboonkerd, V, van Steijn, V, Verhaegh, G, Webber, J P, Witwer, K W, Yuen, P S T, Lei, Z, Llorente, A & Martens-Uzunova, E S 2021, ' Urinary Extracellular Vesicles: A Position Paper by the Urine Task Force of the International Society for Extracellular Vesicles ', Journal of Extracellular Vesicles . https://doi.org/10.1002/jev2.12093, Journal of Extracellular Vesicles, e12093, Journal of Extracellular Vesicles, Vol 10, Iss 7, Pp n/a-n/a (2021), Erdbrügger, U, Blijdorp, C J, Bijnsdorp, I V, Borràs, F E, Burger, D, Bussolati, B, Byrd, J B, Clayton, A, Dear, J W, Falcón-Pérez, J M, Grange, C, Hill, A F, Holthöfer, H, Hoorn, E J, Jenster, G, Jimenez, C R, Junker, K, Klein, J, Knepper, M A, Koritzinsky, E H, Luther, J M, Lenassi, M, Leivo, J, Mertens, I, Musante, L, Oeyen, E, Puhka, M, van Royen, M E, Sánchez, C, Soekmadji, C, Thongboonkerd, V, van Steijn, V, Verhaegh, G, Webber, J P, Witwer, K, Yuen, P S T, Zheng, L, Llorente, A & Martens-Uzunova, E S 2021, ' Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles ', Journal of Extracellular Vesicles, vol. 10, no. 7, e12093 . https://doi.org/10.1002/jev2.12093, Journal of Extracellular Vesicles, 10(7):e12093. Taylor & Francis Ltd, Journal of Extracellular Vesicles, 10, 7, r-IGTP. Repositorio Institucional de Producción Científica del Instituto de Investigación Germans Trias i Pujol, instname, Journal of Extracellular Vesicles, 10(7):e12093. Taylor and Francis Ltd., Journal of Extracellular Vesicles, 10, Journal of Extracellular Vesicles, 10(7)
- Publication Year :
- 2021
-
Abstract
- Urine is commonly used for clinical diagnosis and biomedical research. The discovery of extracellular vesicles (EV) in urine opened a new fast-growing scientific field. In the last decade urinary extracellular vesicles (uEVs) were shown to mirror molecular processes as well as physiological and pathological conditions in kidney, urothelial and prostate tissue. Therefore, several methods to isolate and characterize uEVs have been developed. However, methodological aspects of EV separation and analysis, including normalization of results, need further optimization and standardization to foster scientific advances in uEV research and a subsequent successful translation into clinical practice. This position paper is written by the Urine Task Force of the Rigor and Standardization Subcommittee of ISEV consisting of nephrologists, urologists, cardiologists and biologists with active experience in uEV research. Our aim is to present the state of the art and identify challenges and gaps in current uEV-based analyses for clinical applications. Finally, recommendations for improved rigor, reproducibility and interoperability in uEV research are provided in order to facilitate advances in the field. ESM-U, CG, GV, GJ, IVB, MvR, and VvS, are members of the “IMMPROVE” consortium (Innovative Measurements and Markers for Prostate Cancer Diagnosis and Prognosis using Extracellular Vesicles), which is sponsored by an Alpe d'HuZes grant of the Dutch Cancer Society (grant #EMCR2015-8022). AL is supported by Norges Forskningsråd, Kreftforeningen and Helse Sør-Øst RHF (NO). UE is supported by the NIH, National Heart, Lung, and Blood Institute, Award number K23-HL-126101. CJB and EJH are supported by the Dutch Kidney Foundation (Nierstichting), Award number: CP18.05.
- Subjects :
- 0301 basic medicine
CLINICAL-APPLICATIONS
Urine
0302 clinical medicine
Medicine
Biomarker discovery
Urinary Tract
bladder
biobank
biomarkers
extracellular vesicles
kidney
liquid biopsy
prostate
rigor and standardization
urine
DEEP SEQUENCING ANALYSIS
Prostate
TAMM-HORSFALL PROTEIN
Reference Standards
Extracellular vesicles
BIOMARKER DISCOVERY
Body Fluids
3. Good health
PROSTATE-CANCER
Clinical Practice
030220 oncology & carcinogenesis
Position Paper
PROTEOMIC ANALYSIS
MESSENGER-RNA
MEMBRANE-VESICLES
Histology
Urinary system
Advisory Committees
Scientific field
Bladders
DIABETIC-NEPHROPATHY
03 medical and health sciences
All institutes and research themes of the Radboud University Medical Center
Urological cancers Radboud Institute for Molecular Life Sciences [Radboudumc 15]
Humans
Biology
QH573-671
Task force
business.industry
Reproducibility of Results
Kidneys
Cell Biology
Biobanks
SODIUM-CHLORIDE COTRANSPORTER
030104 developmental biology
Clinical diagnosis
Position paper
1182 Biochemistry, cell and molecular biology
Human medicine
Societies
business
Cytology
Position Papers
Neuroscience
Biomarkers
Subjects
Details
- Language :
- English
- ISSN :
- 20013078
- Database :
- OpenAIRE
- Journal :
- Erdbrügger, U, Blijdorp, C J, Bijnsdorp, I V, Borràs, F E, Burger, D, Bussolati, B, Byrd, J B, Clayton, A, Dear, J W, Falcón-Pérez, J M, Grange, C, Hill, A F, Holthöfer, H, Hoorn, E J, Jenster, G, Jimenez, C R, Junker, K, Klein, J, Knepper, M A, Koritzinsky, E H, Luther, J M, Lenassi, M, Leivo, J, Mertens, I, Musante, L, Oeyen, E, Puhka, M, van Royen, M E, Sánchez, C, Soekmadji, C, Thongboonkerd, V, van Steijn, V, Verhaegh, G, Webber, J P, Witwer, K W, Yuen, P S T, Lei, Z, Llorente, A & Martens-Uzunova, E S 2021, ' Urinary Extracellular Vesicles: A Position Paper by the Urine Task Force of the International Society for Extracellular Vesicles ', Journal of Extracellular Vesicles . https://doi.org/10.1002/jev2.12093, Journal of Extracellular Vesicles, e12093, Journal of Extracellular Vesicles, Vol 10, Iss 7, Pp n/a-n/a (2021), Erdbrügger, U, Blijdorp, C J, Bijnsdorp, I V, Borràs, F E, Burger, D, Bussolati, B, Byrd, J B, Clayton, A, Dear, J W, Falcón-Pérez, J M, Grange, C, Hill, A F, Holthöfer, H, Hoorn, E J, Jenster, G, Jimenez, C R, Junker, K, Klein, J, Knepper, M A, Koritzinsky, E H, Luther, J M, Lenassi, M, Leivo, J, Mertens, I, Musante, L, Oeyen, E, Puhka, M, van Royen, M E, Sánchez, C, Soekmadji, C, Thongboonkerd, V, van Steijn, V, Verhaegh, G, Webber, J P, Witwer, K, Yuen, P S T, Zheng, L, Llorente, A & Martens-Uzunova, E S 2021, ' Urinary extracellular vesicles: A position paper by the Urine Task Force of the International Society for Extracellular Vesicles ', Journal of Extracellular Vesicles, vol. 10, no. 7, e12093 . https://doi.org/10.1002/jev2.12093, Journal of Extracellular Vesicles, 10(7):e12093. Taylor & Francis Ltd, Journal of Extracellular Vesicles, 10, 7, r-IGTP. Repositorio Institucional de Producción Científica del Instituto de Investigación Germans Trias i Pujol, instname, Journal of Extracellular Vesicles, 10(7):e12093. Taylor and Francis Ltd., Journal of Extracellular Vesicles, 10, Journal of Extracellular Vesicles, 10(7)
- Accession number :
- edsair.doi.dedup.....c1aef6124e15e20c47bd672dab407d67