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Significance of hepatitis C virus (HCV) viremia in anti-HCV antibody (IgG) positive subjects
- Source :
- Journal of Medical and Scientific Research, Vol 5, Iss 1, Pp 17-20 (2017)
- Publication Year :
- 2017
- Publisher :
- KIMS Foundation and Research Center, 2017.
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Abstract
- Introduction: Hepatitis C is one of the most common bloodborne diseases causing significant morbidity and mortality globally. Objective: This study was designed to evaluate the correlation between 3rd generation ELISA positives for anti-HCV antibodies with real time PCR, among various categories of patients attending KIMS hospital. Methods: This is a hospital based retrospective study at KIMS hospital, Secunderabad, India conducted between August 2013 and July 2015. From laboratory data, 115 patients, found to be anti-HCV antibody positive (done by CLIA method) were included in the study. Real-Time Reverse Transcription-PCR (RT-PCR) for hepatitis C virus (HCV) was done for these patient samples subsequently. Results: Among a total of 115 anti-HCV antibody positive cases, 70(60.86%) were positive and 45(39.13%) were negative by HCV RNA PCR. Conclusion: ELISA assays have many advantages in diagnostic settings including ease of automation, ease of use, relative cost effectiveness and low variability. Additional or confirmatory testing like RT-PCR is often helpful. The HCV RNA test is still regarded as a standard in the detection of active infection and for monitoring treatment.
Details
- Volume :
- 5
- Database :
- OpenAIRE
- Journal :
- Journal of Medical and Scientific Research
- Accession number :
- edsair.doi.dedup.....c1b68ccf2269e9ff01dd66417a03dfac