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Increased serum HMGB1 levels in patients with Henoch-Schönlein purpura
- Source :
- Experimental Dermatology. 23:419-423
- Publication Year :
- 2014
- Publisher :
- Wiley, 2014.
-
Abstract
- High-mobility group box-1 (HMGB1) has been implicated as a pro-inflammatory cytokine in the pathogenesis of various inflammatory and autoimmune diseases. However, information about HMGB1 in Henoch-Schönlein purpura (HSP) is still unclear. Herein, we investigated the role of HMGB1 in patients with HSP and the pro-inflammatory effects of HMGB1 on human dermal microvascular endothelial cell line (HMEC-1). Serum HMGB1 levels in patients with HSP together with patients with allergic vasculitis (AV) and urticarial vasculitis (UV) were detected by enzyme-linked immunosorbent assay (ELISA). HMEC-1 cells were treated with HMGB1 at concentrations ranging from 4 ng/ml to 100 ng/ml. Serum HMGB1 levels were significantly increased in patients with HSP, AV and UV, when compared with those in control group. Moreover, abundant cytoplasmic expression of HMGB1 was observed in endothelial cells in lesional skin of HSP patients. Using membrane cytokine antibody array, we indicate that HMGB1 markedly induced TNF-α and IL-6 release in cultured supernatant. Furthermore, by real-time quantitative PCR and ELISA, the effects of HMGB1 on these cytokines production in HMEC-1 cells were established. Finally, Western blot data revealed that HMGB1 can induce phosphorylation of inhibitor of κB-α (IκBα) and the nuclear translocation of nuclear factor-κB (NF-κB) p65 in HMEC-1 cells. In conclusion, this study provides first observations on the association of HMGB1 with HSP. We suggest that HMGB1 may be an important mediator of endothelial inflammation through the induction of TNF-α and IL-6 production and may play a crucial role in the pathogenesis of HSP.
- Subjects :
- Adult
Male
Henoch-Schonlein purpura
Adolescent
IgA Vasculitis
Urticaria
medicine.medical_treatment
chemical and pharmacologic phenomena
Dermatology
Biology
Biochemistry
Pathogenesis
Young Adult
Western blot
medicine
Humans
Cytokine Antibody
HMGB1 Protein
Child
Urticarial vasculitis
Molecular Biology
Cells, Cultured
Dose-Response Relationship, Drug
medicine.diagnostic_test
Interleukin-6
Tumor Necrosis Factor-alpha
NF-kappa B
medicine.disease
Endothelial stem cell
IκBα
Cytokine
Case-Control Studies
Immunology
Vasculitis, Leukocytoclastic, Cutaneous
Female
Endothelium, Vascular
Biomarkers
Subjects
Details
- ISSN :
- 09066705
- Volume :
- 23
- Database :
- OpenAIRE
- Journal :
- Experimental Dermatology
- Accession number :
- edsair.doi.dedup.....c1d2e6b46714963a990d2de3f1d2424c
- Full Text :
- https://doi.org/10.1111/exd.12422