Back to Search
Start Over
Lycium barbarum Polysaccharide Prevents Focal Cerebral Ischemic Injury by Inhibiting Neuronal Apoptosis in Mice
- Source :
- PLoS ONE, PLoS ONE, Vol 9, Iss 3, p e90780 (2014)
- Publication Year :
- 2014
- Publisher :
- Public Library of Science, 2014.
-
Abstract
- Aims of the Study To investigate the neuroprotective effect of Lycium barbarum polysaccharide (LBP) on focal cerebral ischemic injury in mice and to explore its possible mechanism. Materials and Methods Male ICR mice were used to make the model of middle cerebral artery occlusion (MCAO) after intragastric administration with LBP (10, 20 and 40 mg/kg) and Nimodipine (0.4 mg/kg) for seven successive days. After 24 h of reperfusion, neurological scores were estimated and infarct volumes were measured by 2, 3, 5-triphenyltetrazolium chloride (TTC) staining. Morphological changes in ischemic brains were performed for hematoxylin-eosin (HE) staining. The number of apoptotic neurons was detected by TUNEL staining. The Bax, Bcl-2 protein expression and CytC, Caspase-3, -9 and cleaved PARP-1 activation were investigated by immunofluorescence and western-blot analysis. Results LBP (10, 20 and 40 mg/kg) treatment groups significantly reduced infract volume and neurological deficit scores. LBP also relieved neuronal morphological damage and attenuated the neuronal apoptosis. LBP at the dose of 40 mg/kg significantly suppressed overexpression of Bax, CytC, Caspase-3, -9 and cleaved PARP-1, and inhibited the reduction of Bcl-2 expression. Conclusions Based on these findings we propose that LBP protects against focal cerebral ischemic injury by attenuating the mitochondrial apoptosis pathway.
- Subjects :
- Male
Mouse
Poly (ADP-Ribose) Polymerase-1
lcsh:Medicine
Fluorescent Antibody Technique
Apoptosis
Pharmacology
Biochemistry
Brain Ischemia
Brain ischemia
Mice
Drug Discovery
Molecular Cell Biology
Signaling in Cellular Processes
lcsh:Science
Apoptotic Signaling Cascade
Apoptotic Signaling
Drug Dependence
Neurons
Mice, Inbred ICR
Multidisciplinary
TUNEL assay
Neuromodulation
Neurochemistry
Neurodegenerative Diseases
Infarction, Middle Cerebral Artery
Neurotransmitters
Animal Models
Signaling Cascades
Drug Marketing
Blot
Dose–response relationship
Chemistry
Cerebellar Disorders
Neurology
Behavioral Pharmacology
Middle cerebral artery
Medicine
Neurochemicals
Poly(ADP-ribose) Polymerases
medicine.drug
Research Article
Signal Transduction
Drugs and Devices
Drug Research and Development
Blotting, Western
Neuroprotection
Protein Chemistry
Model Organisms
Neuropharmacology
medicine.artery
Chemical Biology
medicine
In Situ Nick-End Labeling
Animals
Theoretical Chemistry
Nimodipine
Biology
Analysis of Variance
Dose-Response Relationship, Drug
business.industry
Cerebral Palsy
lcsh:R
Proteins
medicine.disease
Molecular biology
lcsh:Q
business
Drugs, Chinese Herbal
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 9
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....c1e9b514f480b0037db801cfd18bbcf7