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Elevated MTSS1 expression associated with metastasis and poor prognosis of residual hepatitis B-related hepatocellular carcinoma
- Source :
- Journal of Experimental & Clinical Cancer Research : CR
- Publication Year :
- 2016
- Publisher :
- BioMed Central, 2016.
-
Abstract
- Background Hepatectomy generally offers the best chance of long-term survival for patients with hepatocellular carcinoma (HCC). Many studies have shown that hepatectomy accelerates tumor metastasis, but the mechanism remains unclear. Methods An orthotopic nude mice model with palliative HCC hepatectomy was performed in this study. Metastasis-related genes in tumor following resection were screened; HCC invasion, metastasis, and some molecular alterations were examined in vivo and in vitro. Clinical significance of key gene mRNA expression was also analyzed. Results Metastasis suppressor 1 (MTSS1) located in the central position of gene function net of residual HCC. MTSS1 was up-regulated in residual tumor after palliative resection. In hepatitis B-related HCC patients undergone palliative hepatectomy, those with higher MTSS1 mRNA expression accompanied by activation of matrix metalloproteinase 2 (MMP2) in residual HCC, had earlier residual HCC detection after hepatectomy and poorer survival when compared to those with lower MTSS1. In different cell lines, the levels of MTSS1 mRNA increased in parallel with metastatic potential. MTSS1 down regulation via siRNA decreased MMP2 activity, reduced invasive potentials of HCC by 28.9 % in vitro, and averted the deteriorated lung metastatic extent in vivo. Conclusions The poor prognosis of hepatitis B-related HCC patients following palliative hepatectomy associates with elevated MTSS1 mRNA expression; therefore, MTSS1 may provide a new research field for HCC diagnosis and treatment. Electronic supplementary material The online version of this article (doi:10.1186/s13046-016-0361-8) contains supplementary material, which is available to authorized users.
- Subjects :
- 0301 basic medicine
Oncology
Male
Cancer Research
MMP2
Lung Neoplasms
Hepatocellular carcinoma
medicine.medical_treatment
Metastasis
Mice
0302 clinical medicine
Invasion
Neoplasm Metastasis
Liver Neoplasms
Microfilament Proteins
Hepatitis B
Prognosis
Neoplasm Proteins
Up-Regulation
Gene Expression Regulation, Neoplastic
Treatment Outcome
030220 oncology & carcinogenesis
Matrix Metalloproteinase 2
Female
Metastasis suppressor 1
Erratum
medicine.medical_specialty
Carcinoma, Hepatocellular
Mice, Nude
03 medical and health sciences
In vivo
Internal medicine
Cell Line, Tumor
medicine
Animals
Humans
Survival analysis
Hepatitis
business.industry
Research
medicine.disease
Survival Analysis
digestive system diseases
030104 developmental biology
Hepatectomy
business
Neoplasm Transplantation
Subjects
Details
- Language :
- English
- ISSN :
- 17569966 and 03929078
- Volume :
- 35
- Database :
- OpenAIRE
- Journal :
- Journal of Experimental & Clinical Cancer Research : CR
- Accession number :
- edsair.doi.dedup.....c205380a4ec78369fc52c96a0c33264e