Supplementary Data from Transcriptional Regulation of CXCR4 in Prostate Cancer: Significance of TMPRSS2-ERG Fusions

Supplementary Data - PDF file 589K, S1. Mutations in putative Ets/ERG binding sites in CXCR4 promoter oigos diminishes oligo binding with either VCaP cell nuclear extracts or in vitro translated ERG protein. S2. ERG ChIP seq analysis of VCaP cells. S3. N-terminus deletion of ERG regulates CXCR4 promoter activation similar to full length ERG protein. S4. LNCaP cells were transfected with ARR2Pb-LUC as a vector control and ARR2Pb-ERG-LUC plasmid. S5. MS3 spectrum of trypsin-digested, TiO2 eluted ERG peptides from VCaP cells