Back to Search
Start Over
Combination of inductive effect of lipopolysaccharide and in situ mechanical conditioning for forming an autologous vascular graft in vivo
- Source :
- Scientific Reports, Scientific Reports, Vol 9, Iss 1, Pp 1-17 (2019)
- Publication Year :
- 2019
- Publisher :
- Springer Science and Business Media LLC, 2019.
-
Abstract
- Autologous vascular grafts have the advantages of better biocompatibility and prognosis. However, previous studies that implanted bare polymer tubes in animals to grow autologous tubular tissues were limited by their poor yield rates and stability. To enhance the yield rate of the tubular tissue, we employed a design with the addition of overlaid autologous whole blood scaffold containing lipopolysaccharides (LPS). Furthermore, we applied in vivo dynamic mechanical stimuli through cyclically inflatable silicone tube to improve the mechanical properties of the harvested tissues. The effectiveness of the modification was examined by implanting the tubes in the peritoneal cavity of rats. A group without mechanical stimuli served as the controls. After 24 days of culture including 16 days of cyclic mechanical stimuli, we harvested the tubular tissue forming on the silicone tube for analysis or further autologous interposition vascular grafting. In comparison with those without cyclic dynamic stimuli, tubular tissues with this treatment during in vivo culture had stronger mechanical properties, better smooth muscle differentiation, and more collagen and elastin expression by the end of incubation period in the peritoneal cavity. The grafts remained patent after 4 months of implantation and showed the presence of endothelial and smooth muscle cells. This model shows a new prospect for vascular tissue engineering.
- Subjects :
- Lipopolysaccharides
Male
0301 basic medicine
In situ
Scaffold
Lipopolysaccharide
Biocompatibility
Blotting, Western
Silicones
lcsh:Medicine
Article
Rats, Sprague-Dawley
03 medical and health sciences
chemistry.chemical_compound
Peritoneal cavity
0302 clinical medicine
In vivo
medicine
Animals
Tissue engineering
lcsh:Science
Autografts
Aorta
Ultrasonography
Multidisciplinary
Tissue Scaffolds
biology
Chemistry
lcsh:R
Elastin
Rats
030104 developmental biology
medicine.anatomical_structure
Regenerative medicine
biology.protein
Conditioning
lcsh:Q
Vascular Grafting
Collagen
030217 neurology & neurosurgery
Biomedical engineering
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....c248dd04b821476cd96e5ac3bcc37da1