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EA-D p45-IgG as a potential biomarker for nasopharyngeal carcinoma diagnosis
- Source :
- Asian Pacific journal of cancer prevention : APJCP. 14(12)
- Publication Year :
- 2014
-
Abstract
- Aim: To identify new biomarkers for NPC diagnosis with an anti-EBV Western blot test kit. Methods: Serum samples from 64 NPC patients and healthy subjects with four specific VCA-IgA/EA-IgA profiles were tested with an anti-EBV Western blot test kit from EUROIMMUN AG. Proteins were quantified with scores of intensity visually assigned to the protein bands. The markers which showed statistical differences between the NPC and non-NPC subjects were further evaluated in another 32 NPC patients and 32 controls in comparison with established biomarkers including VCA-IgA, EA-IgA, EBV-related protein IgG, and EBV DNA. Results: Among the markers screened, EA-D p45-IgG showed a statistically significant difference ( p < 0.05) between NPC and non-NPC subjects with VCA-IgA positivy. In 32 VCA-IgA positive NPC patients and 32 control subjects, the diagnostic accuracy of EA-D p45-IgG was 78.1% with a positive predictive value of 77.8% and a negative predictive value of 78.6%. In the verification experiment, the specificity and sensitivity of EA-D p45-IgG were 75.0% and 90.6 %, respectively. Conclusions: EA-D p45-IgG might be a potential biomarker for NPC diagnosis, especially among VCA-IgA positive subjects.
- Subjects :
- Adult
Male
Cancer Research
Pathology
medicine.medical_specialty
Herpesvirus 4, Human
Epidemiology
Enzyme-Linked Immunosorbent Assay
Biology
Antibodies, Viral
Gastroenterology
Western blot test
Western blot
Internal medicine
otorhinolaryngologic diseases
medicine
Biomarkers, Tumor
Humans
Antigens, Viral
Aged
Neoplasm Staging
medicine.diagnostic_test
Significant difference
Public Health, Environmental and Occupational Health
Healthy subjects
Nasopharyngeal Neoplasms
Middle Aged
medicine.disease
Serum samples
Control subjects
Prognosis
stomatognathic diseases
Oncology
Nasopharyngeal carcinoma
ROC Curve
Potential biomarkers
Case-Control Studies
Immunoglobulin G
Capsid Proteins
Female
Follow-Up Studies
Subjects
Details
- ISSN :
- 2476762X
- Volume :
- 14
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- Asian Pacific journal of cancer prevention : APJCP
- Accession number :
- edsair.doi.dedup.....c25f5340c48208e036efb68215c5fec4