Back to Search
Start Over
Point-of-care SARS-CoV-2 serological assays for enhanced case finding in a UK inpatient population
- Source :
- Scientific Reports, Vol 11, Iss 1, Pp 1-8 (2021), Scientific Reports
- Publication Year :
- 2021
- Publisher :
- Nature Portfolio, 2021.
-
Abstract
- Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2) has become a global pandemic. Case identification is currently made by real-time polymerase chain reaction (PCR) during the acute phase and largely restricted to healthcare laboratories. Serological assays are emerging but independent validation is urgently required to assess their utility. We evaluated five different point-of-care (POC) SARS-CoV-2 antibody test kits against PCR, finding concordance across the assays (n = 15). We subsequently tested 200 patients using the OrientGene COVID-19 IgG/IgM Rapid Test Cassette and find a sensitivity of 74% in the early infection period (day 5–9 post symptom onset), with 100% sensitivity not seen until day 13, demonstrating inferiority to PCR testing in the infectious period. Negative rate was 96%, but in validating the serological tests uncovered potential false-negatives from PCR testing late-presenting cases. A positive predictive value (PPV) of 37% in the general population precludes any use for general screening. Where a case definition is applied however, the PPV is substantially improved (95.4%), supporting use of serology testing in carefully targeted, high-risk populations. Larger studies in specific patient cohorts, including those with mild infection are urgently required to inform on the applicability of POC serological assays to help control the spread of SARS-CoV-2 and improve case finding of patients that may experience late complications.
- Subjects :
- Male
0301 basic medicine
Antibodies, Viral
law.invention
Serology
COVID-19 Testing
0302 clinical medicine
law
Medicine
Public Health Surveillance
030212 general & internal medicine
Polymerase chain reaction
Aged, 80 and over
education.field_of_study
Multidisciplinary
biology
Middle Aged
Real-time polymerase chain reaction
Point-of-Care Testing
Female
medicine.medical_specialty
Point-of-care testing
Concordance
Science
030106 microbiology
Population
Real-Time Polymerase Chain Reaction
Article
03 medical and health sciences
Virology
Internal medicine
Humans
Serologic Tests
Clinical microbiology
education
Aged
Point of care
Inpatients
SARS-CoV-2
business.industry
Infectious-disease diagnostics
COVID-19
Reproducibility of Results
United Kingdom
Immunoglobulin M
Immunoglobulin G
biology.protein
business
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Volume :
- 11
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....c2853b21e7e0249421def733b42c304b