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Removal of prothioconazole using screened microorganisms and identification of biodegradation products via UPLC-QqTOF-MS
- Source :
- Ecotoxicology and environmental safety. 206
- Publication Year :
- 2020
-
Abstract
- Degradation of the prothioconazole by three strains of microorganisms isolated from activated sludge obtained from a pesticide factory was assessed, and an ultrahigh-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QqTOF-MS) method for the determination of prothioconazole and its metabolites was established. The optimal conditions for the degradation of prothioconazole were determined by single factor optimization experiments. A degradation rate of 93.32% is achieved when the prothioconazole is co-cultured with the strain W313 at a cultivation time of 60 h, a cultivation temperature of 30 °C, a pH of 6.33, a prothioconazole concentration of 50 mg L−1, a microorganism volume of 10%, and a dextrose volume of 4%. The three effective microorganism strains were identified by morphological and molecular biology to be Candida tropicalis, Enterobacter cloacae, and Pseudomonas aeruginosa. UPLC-QqTOF-MS analysis allowed the identification of 62 different prothioconazole degradation products produced by the strain cultures, with prothioconazole-desthio, prothioconazole-dechloropropyl, and oxidizing prothioconazole being the main products. In addition, degradation products from different strains and conditions were compared. The results of scatter plot (S-Plot) analysis indicated that C9H7NO, C10H17N7, and C12H13ClN2O were only detected in the products incubated with Enterobacter cloacae. Thus, this study demonstrates that Enterobacter cloacae and Pseudomonas aeruginosa possesses high potential for bioremediation of prothioconazole-contaminated environments.
- Subjects :
- Health, Toxicology and Mutagenesis
Microorganism
0211 other engineering and technologies
02 engineering and technology
010501 environmental sciences
Effective microorganism
medicine.disease_cause
01 natural sciences
Mass Spectrometry
Candida tropicalis
Bioremediation
Enterobacter cloacae
medicine
Pesticides
Chromatography, High Pressure Liquid
0105 earth and related environmental sciences
021110 strategic, defence & security studies
Chromatography
biology
Sewage
Pseudomonas aeruginosa
Chemistry
Public Health, Environmental and Occupational Health
General Medicine
Biodegradation
Models, Theoretical
Triazoles
biology.organism_classification
Pollution
Activated sludge
Biodegradation, Environmental
Subjects
Details
- ISSN :
- 10902414
- Volume :
- 206
- Database :
- OpenAIRE
- Journal :
- Ecotoxicology and environmental safety
- Accession number :
- edsair.doi.dedup.....c2b42ccae3f49f71f664a74942b079cb