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An outbreak of neonatal enteritis in buffalo calves associated with astrovirus
- Source :
- Journal of Veterinary Science
- Publication Year :
- 2021
-
Abstract
- Background: Enteritis of an infectious origin is a major cause of productivity and economic losses to cattle producers worldwide. Several pathogens are believed to cause or contribute to the development of calf diarrhea. Astroviruses (AstVs) are neglected enteric pathogens in ruminants, but they have recently gained attention because of their possible association with encephalitis in humans and various animal species, including cattle. Objectives: This paper describes a large outbreak of neonatal diarrhea in buffalo calves (Bubalus bubalis), characterized by high mortality, which was associated with an AstV infection. Methods: Following an enteritis outbreak characterized by high morbidity (100%) and mortality (46.2%) in a herd of Mediterranean buffaloes (B. bubalis) in Italy, 16 samples from buffalo calves were tested with the molecular tools for common and uncommon enteric pathogens, including AstV, kobuvirus, and torovirus. Results: The samples tested negative for common enteric viral agents, including Rotavirus A, coronavirus, calicivirus, pestivirus, kobuvirus, and torovirus, while they tested positive for AstV. Overall, 62.5% (10/16) of the samples were positive in a single round reverse transcription polymerase chain reaction (PCR) assay for AstV, and 100% (16/16) were positive when nested PCR was performed. The strains identified in the outbreak showed a clonal origin and shared the closest genetic relationship with bovine AstVs (up to 85% amino acid identity in the capsid). Conclusions: This report indicates that AstVs should be included in a differential diagnosis of infectious diarrhea in buffalo calves.
- Subjects :
- Gene Expression Regulation, Viral
Buffaloes
Torovirus
Buffalo
medicine.disease_cause
astroviridae
Gene Expression Regulation, Enzymologic
Astrovirus
Enteritis
Disease Outbreaks
Rotavirus
Astroviridae Infections
Virology
Astroviridae Infection
medicine
Animals
Phylogeny
Disease Outbreak
General Veterinary
biology
enteriti
Animal
Pestivirus
Outbreak
biology.organism_classification
medicine.disease
Buffaloe
RNA-Dependent RNA Polymerase
Capsid Protein
infection
Diarrhea
Animals, Newborn
Italy
Kobuvirus
Capsid Proteins
Original Article
medicine.symptom
Subjects
Details
- ISSN :
- 1976555X
- Volume :
- 22
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Journal of veterinary science
- Accession number :
- edsair.doi.dedup.....c2cc830657bcd12c2cbb7bb4d46ea5b7