Additional file 3: of Generation of populations of antigen-specific cytotoxic T cells using DCs transfected with DNA construct encoding HER2/neu tumor antigen epitopes

Typical scatter plots of the experimental sample showing the gating scheme. The experimental sample is the propidium iodide (PI)-labeled coculture of MCF-7 cells and HER2-specific cells. A – Scatter plot showing the distribution of all events corresponding to the emission parameters of the CFSE label in the FITC channel. Events corresponding CFSE-labeled cells are gated. B – Scatter plot showing the distribution of events from region of CFSE-labeled cells. Events corresponding to MCF-7 cells in terms of their phenotypic parameters (forward and side light scattering) are gated. C – Scatter plot showing the distribution of all events in the experimental sample with respect to parameters of forward and side light scattering. D – Scatter plot of the experimental sample (PI-labeled co-culture of MCF-7 cells and HER2-specific cells). E – Scatter plot of the control over spontaneous death of target cells (PI-labeled MCF-7 cells). (DOCX 160 kb)