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Effect of Exogenous Fetuin-A on TGF-β/Smad Signaling in Hepatic Stellate Cells
Effect of Exogenous Fetuin-A on TGF-β/Smad Signaling in Hepatic Stellate Cells
- Source :
- BioMed Research International, Vol 2016 (2016), BioMed Research International
- Publication Year :
- 2016
- Publisher :
- Hindawi Publishing Corporation, 2016.
-
Abstract
- Objective. To explore the effects of low concentration of exogenous fetuin-A intervention on TGF-β1 induced LX2 cells through detection of the expression of mRNA and protein of Smad2, Smad3, and Smad7. Methods. MTT assay was used to detect the LX2 cells proliferation and the regression equation calculating software was applied to determine IC50 of fetuin-A. RT-PCR was used to determine the relative content of Smad2, Smad3, and Smad7 mRNA in LX2 cells. Western blot was used to detect the LX2 cells relative content of Smad2, Smad3, Smad7 protein expression, respectively. Results. The analysis from RT-PCR and western blot showed that when compared with the other groups TGF-β1 + fetuin-A group increased the expression of Smad2 and Smad3 while decreased the expression of Smad7 (P<0.05). Conclusion. Fetuin-A may improve the excessive activation of hepatic stellate cells which is caused by an enhanced positive regulation of Smad2 and Smad3 protein and the deficiency in negative regulation of Smad7 protein. This is through inhibiting the expression of Smad2 and Smad3 gene and promoting the expression of Smad7 gene. As a result, the development of liver fibrosis will be reduced.
- Subjects :
- Liver Cirrhosis
0301 basic medicine
Article Subject
alpha-2-HS-Glycoprotein
Gene Expression
lcsh:Medicine
Smad Proteins
Smad2 Protein
Biology
General Biochemistry, Genetics and Molecular Biology
Cell Line
Smad7 Protein
03 medical and health sciences
Western blot
Transforming Growth Factor beta
Hepatic Stellate Cells
medicine
Humans
MTT assay
RNA, Messenger
Smad3 Protein
Gene
IC50
Cell Proliferation
Messenger RNA
General Immunology and Microbiology
medicine.diagnostic_test
integumentary system
lcsh:R
General Medicine
Fetuin
Molecular biology
030104 developmental biology
Cell culture
Hepatic stellate cell
biological phenomena, cell phenomena, and immunity
Research Article
Signal Transduction
Subjects
Details
- Language :
- English
- ISSN :
- 23146133
- Database :
- OpenAIRE
- Journal :
- BioMed Research International
- Accession number :
- edsair.doi.dedup.....c30301edefd2a698b3b35ee3f37cb7cb
- Full Text :
- https://doi.org/10.1155/2016/8462615