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Biodegradable composite porous poly(dl-lactide-co-glycolide) scaffold supports mesenchymal stem cell differentiation and calcium phosphate deposition
- Publication Year :
- 2017
- Publisher :
- Taylor & Francis, 2017.
-
Abstract
- In recent decades, tissue engineering strategies have been proposed for the treatment of musculoskeletal diseases and bone fractures to overcome the limitations of the traditional surgical approaches based on allografts and autografts. In this work we report the development of a composite porous poly(DL-lactide-co-glycolide) scaffold suitable for bone regeneration. Scaffolds were produced by thermal sintering of porous microparticles. Next, in order to improve cell adhesion to the scaffold and subsequent proliferation, the scaffolds were coated with the osteoconductive biopolymers chitosan and sodium alginate, in a process that exploited electrostatic interactions between the positively charged biopolymers and the negatively charged PLGA scaffold. The resulting scaffolds were characterized in terms of porosity, degradation rate, mechanical properties, biocompatibility and suitability for bone regeneration. They were found to have an overall porosity of approximate to 85% and a degradation half time of approximate to 2weeks, considered suitable to support de novo bone matrix deposition from mesenchymal stem cells. Histology confirmed the ability of the scaffold to sustain adipose-derived mesenchymal stem cell adhesion, infiltration, proliferation and osteo-differentiation. Histological staining of calcium and microanalysis confirmed the presence of calcium phosphate in the scaffold sections.
- Subjects :
- Calcium Phosphates
0301 basic medicine
Scaffold
Calcium Phosphate
adipose-derived mesenchymal stem cell
Biomedical Engineering
Pharmaceutical Science
Medicine (miscellaneous)
chemistry.chemical_element
02 engineering and technology
Calcium
Chitosan
03 medical and health sciences
chemistry.chemical_compound
Tissue engineering
Osteogenesis
Microparticle sintering
Humans
alginate
bone tissue engineering
Porosity
Polyglactin 910
Mechanical Phenomena
adipose-derived mesenchymal stem cells
Chemistry
Osteogenesi
Cell Differentiation
Mesenchymal Stem Cells
General Medicine
021001 nanoscience & nanotechnology
chitosan
Biodegradable composites
Mesenchymal Stem Cell
030104 developmental biology
Adipose Tissue
Chemical engineering
Mesenchymal stem cell differentiation
0210 nano-technology
Poly dl lactide co glycolide
Human
Biotechnology
Subjects
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....c319731b027f6a4b8322d2254e3f3092
- Full Text :
- https://doi.org/10.6084/m9.figshare.5727037