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The sequence and de novo assembly of the giant panda genome
- Source :
- Li, R, Fan, W, Tian, G, Zhu, H, He, L, Cai, J, Huang, Q, Cai, Q, Li, B, Bai, Y, Zhang, Z, Zhang, Y, Wang, W, Li, J, Wei, F, Li, H, Jian, M, Li, J, Zhang, Z, Nielsen, R, Li, D, Gu, W, Yang, Z, Xuan, Z, Ryder, O A, Leung, F C-C, Zhou, Y, Cao, J, Sun, X, Fu, Y, Fang, X, Guo, X, Wang, B, Hou, R, Shen, F, Mu, B, Ni, P, Lin, R, Qian, W, Wang, G, Yu, C, Nie, W, Wang, J, Wu, Z, Liang, H, Min, J, Wu, Q, Cheng, S, Ruan, J, Wang, M, Shi, Z, Wen, M, Liu, B, Ren, X, Zheng, H, Dong, D, Cook, K, Shan, G, Zhang, H, Kosiol, C, Xie, X, Lu, Z, Zheng, H, Li, Y, Steiner, C C, Lam, T T-Y, Lin, S, Zhang, Q, Li, G, Tian, J, Gong, T, Liu, H, Zhang, D, Fang, L, Ye, C, Zhang, J, Hu, W, Xu, A, Ren, Y, Zhang, G, Bruford, M W, Li, Q, Ma, L, Guo, Y, An, N, Hu, Y, Zheng, Y, Shi, Y, Li, Z, Liu, Q, Chen, Y, Zhao, J, Qu, N, Zhao, S, Tian, F, Wang, X, Wang, H, Xu, L, Liu, X, Vinar, T, Wang, Y, Lam, T-W, Yiu, S-M, Liu, S, Zhang, H, Li, D, Huang, Y, Wang, X, Yang, G, Jiang, Z, Wang, J, Qin, N, Li, L, Li, J, Bolund, L, Kristiansen, K, Wong, G K-S, Olson, M, Zhang, X, Li, S, Yang, H, Wang, J & Wang, J 2009, ' The sequence and de novo assembly of the giant panda genome ', Nature, vol. 463, no. 7279, pp. 311-7 . https://doi.org/10.1038/nature08696
- Publication Year :
- 2009
-
Abstract
- Using next-generation sequencing technology alone, we have successfully generated and assembled a draft sequence of the giant panda genome. The assembled contigs (2.25 gigabases (Gb)) cover approximately 94% of the whole genome, and the remaining gaps (0.05 Gb) seem to contain carnivore-specific repeats and tandem repeats. Comparisons with the dog and human showed that the panda genome has a lower divergence rate. The assessment of panda genes potentially underlying some of its unique traits indicated that its bamboo diet might be more dependent on its gut microbiome than its own genetic composition. We also identified more than 2.7 million heterozygous single nucleotide polymorphisms in the diploid genome. Our data and analyses provide a foundation for promoting mammalian genetic research, and demonstrate the feasibility for using next-generation sequencing technologies for accurate, cost-effective and rapid de novo assembly of large eukaryotic genomes. © 2010 Macmillan Publishers Limited. All rights reserved.<br />link_to_OA_fulltext
- Subjects :
- Cancer genome sequencing
Heterozygote
China
Sequence assembly
Receptors, G-Protein-Coupled - genetics
Genomics
Biology
Genome
Polymorphism, Single Nucleotide
Synteny
Article
Receptors, G-Protein-Coupled
Evolution, Molecular
Contig Mapping
Dogs
Tandem repeat
biology.animal
Animals
Humans
Diet - veterinary
Ursidae - classification - genetics - physiology
Conserved Sequence
Ailuropoda melanoleuca
Genetics
Multidisciplinary
Contig
Sequence Analysis, DNA
Genome - genetics
Polymorphism, Single Nucleotide - genetics
Diet
Fertility
Multigene Family
Multigene Family - genetics
Fertility - genetics - physiology
Synteny - genetics
Female
Sequence Alignment
Ursidae
Algorithms
Reference genome
Conserved Sequence - genetics
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Li, R, Fan, W, Tian, G, Zhu, H, He, L, Cai, J, Huang, Q, Cai, Q, Li, B, Bai, Y, Zhang, Z, Zhang, Y, Wang, W, Li, J, Wei, F, Li, H, Jian, M, Li, J, Zhang, Z, Nielsen, R, Li, D, Gu, W, Yang, Z, Xuan, Z, Ryder, O A, Leung, F C-C, Zhou, Y, Cao, J, Sun, X, Fu, Y, Fang, X, Guo, X, Wang, B, Hou, R, Shen, F, Mu, B, Ni, P, Lin, R, Qian, W, Wang, G, Yu, C, Nie, W, Wang, J, Wu, Z, Liang, H, Min, J, Wu, Q, Cheng, S, Ruan, J, Wang, M, Shi, Z, Wen, M, Liu, B, Ren, X, Zheng, H, Dong, D, Cook, K, Shan, G, Zhang, H, Kosiol, C, Xie, X, Lu, Z, Zheng, H, Li, Y, Steiner, C C, Lam, T T-Y, Lin, S, Zhang, Q, Li, G, Tian, J, Gong, T, Liu, H, Zhang, D, Fang, L, Ye, C, Zhang, J, Hu, W, Xu, A, Ren, Y, Zhang, G, Bruford, M W, Li, Q, Ma, L, Guo, Y, An, N, Hu, Y, Zheng, Y, Shi, Y, Li, Z, Liu, Q, Chen, Y, Zhao, J, Qu, N, Zhao, S, Tian, F, Wang, X, Wang, H, Xu, L, Liu, X, Vinar, T, Wang, Y, Lam, T-W, Yiu, S-M, Liu, S, Zhang, H, Li, D, Huang, Y, Wang, X, Yang, G, Jiang, Z, Wang, J, Qin, N, Li, L, Li, J, Bolund, L, Kristiansen, K, Wong, G K-S, Olson, M, Zhang, X, Li, S, Yang, H, Wang, J & Wang, J 2009, ' The sequence and de novo assembly of the giant panda genome ', Nature, vol. 463, no. 7279, pp. 311-7 . https://doi.org/10.1038/nature08696
- Accession number :
- edsair.doi.dedup.....c31d14f81fbeef40bb598ed832652aec
- Full Text :
- https://doi.org/10.1038/nature08696