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Mesenchymal stem cells secretome-induced axonal outgrowth is mediated by BDNF
- Source :
- Scientific Reports, Repositório Científico de Acesso Aberto de Portugal, Repositório Científico de Acesso Aberto de Portugal (RCAAP), instacron:RCAAP, Scientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
- Publication Year :
- 2017
-
Abstract
- Mesenchymal stem cells (MSCs) have been used for cell-based therapies in regenerative medicine, with increasing importance in central and peripheral nervous system repair. However, MSCs grafting present disadvantages, such as, a high number of cells required for transplantation and low survival rate when transplanted into the central nervous system (CNS). In line with this, MSCs secretome which present on its composition a wide range of molecules (neurotrophins, cytokines) and microvesicles, can be a solution to surpass these problems. However, the effect of MSCs secretome in axonal elongation is poorly understood. In this study, we demonstrate that application of MSCs secretome to both rat cortical and hippocampal neurons induces an increase in axonal length. In addition, we show that this growth effect is axonal intrinsic with no contribution from the cell body. To further understand which are the molecules required for secretome-induced axonal outgrowth effect, we depleted brain-derived neurotrophic factor (BDNF) from the secretome. Our results show that in the absence of BDNF, secretome-induced axonal elongation effect is lost and that axons present a reduced axonal growth rate. Altogether, our results demonstrate that MSCs secretome is able to promote axonal outgrowth in CNS neurons and this effect is mediated by BDNF.
- Subjects :
- 0301 basic medicine
Central Nervous System
Proteome
Science
Medicina Básica [Ciências Médicas]
Central nervous system
Microfluidics
Models, Neurological
Neuronal Outgrowth
Biology
Regenerative medicine
Article
Umbilical Cord
03 medical and health sciences
0302 clinical medicine
Neurotrophic factors
medicine
Animals
Humans
Receptor, trkB
Rats, Wistar
Brain-derived neurotrophic factor
Neurons
Science & Technology
Multidisciplinary
Brain-Derived Neurotrophic Factor
Mesenchymal stem cell
Mesenchymal Stem Cells
Anatomy
Microvesicles
3. Good health
Cell biology
Transplantation
030104 developmental biology
medicine.anatomical_structure
nervous system
Culture Media, Conditioned
biology.protein
Medicine
030217 neurology & neurosurgery
Neurotrophin
Subjects
Details
- ISSN :
- 20452322
- Volume :
- 7
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Scientific reports
- Accession number :
- edsair.doi.dedup.....c31dbe20e5552c382f191b6ee786052f