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Isolation of plasmid pKM101 in the Stocker laboratory
- Source :
- Mutation research. 612(3)
- Publication Year :
- 2006
-
Abstract
- pKM101 is a mutagenesis-enhancing resistance transfer plasmid (R plasmid) that was introduced into several tester strains used in the Salmonella/microsome mutation assay (Ames test). Plasmid pKM101 has contributed substantially to the effectiveness of the Ames assay, which is used on a world-wide basis to detect mutagens and is required by many government regulatory agencies for approval to market new drugs and other chemical agents. Widely used since 1975, the Ames test is still regarded as one of the most sensitive genetic toxicity assays and a useful short-term test for predicting carcinogenicity in animals. Plasmid pKM101, which is a deletion derivative of plasmid R46 (also referred to as R-Brighton after its origin of isolation in Brighton, England), has also been used to elucidate molecular mechanisms of mutagenesis. It was isolated in the laboratory of Professor Bruce A.D. Stocker at Stanford University as part of my doctoral research with 20 R plasmids. Professor Stocker's phenomenal insight into the genetics of Salmonella typhimurium and plasmid behavior was a major factor that led to the isolation of pKM101. This paper includes a tribute to Bruce Stocker, together with a summary of my research with mutagenesis-enhancing R plasmids and a brief discussion of the molecular mechanisms involved in pKM101 plasmid-mediated bacterial mutagenesis.
- Subjects :
- Genetics
Mutation
Salmonella
Carcinogenicity Tests
Mutagenicity Tests
Health, Toxicology and Mutagenesis
R Factors
Mutagenesis (molecular biology technique)
R Plasmids
Biology
History, 20th Century
Isolation (microbiology)
medicine.disease_cause
Plasmid pKM101
Ames test
Microbiology
Plasmid
Mutagenesis
medicine
Plasmids
Subjects
Details
- ISSN :
- 00275107
- Volume :
- 612
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Mutation research
- Accession number :
- edsair.doi.dedup.....c33010c52900e3719f0991efe41dda72