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Developing a MEMS device with built-in microfluidics for biophysical single cell characterization
- Source :
- Micromachines, Micromachines, MDPI, 2018, 9 (6), pp.275. ⟨10.3390/mi9060275⟩, Micromachines, 2018, 9 (6), pp.275. ⟨10.3390/mi9060275⟩, Micromachines, Vol 9, Iss 6, p 275 (2018), Volume 9, Issue 6
- Publication Year :
- 2018
- Publisher :
- HAL CCSD, 2018.
-
Abstract
- This study combines the high-throughput capabilities of microfluidics with the sensitive measurements of microelectromechanical systems (MEMS) technology to perform biophysical characterization of circulating cells for diagnostic purposes. The proposed device includes a built-in microchannel that is probed by two opposing tips performing compression and sensing separately. Mechanical displacement of the compressing tip (up to a maximum of 14 &micro<br />m) and the sensing tip (with a quality factor of 8.9) are provided by two separate comb-drive actuators, and sensing is performed with a capacitive displacement sensor. The device is designed and developed for simultaneous electrical and mechanical measurements. As the device is capable of exchanging the liquid inside the channel, different solutions were tested consecutively. The performance of the device was evaluated by introducing varying concentrations of glucose (from 0.55 mM (0.1%) to 55.5 mM (10%)) and NaCl (from 0.1 mM to 10 mM) solutions in the microchannel and by monitoring changes in the mechanical and electrical properties. Moreover, we demonstrated biological sample handling by capturing single cancer cells. These results show three important capabilities of the proposed device: mechanical measurements, electrical measurements, and biological sample handling. Combined in one device, these features allow for high-throughput multi-parameter characterization of single cells.
- Subjects :
- 0301 basic medicine
Materials science
lcsh:Mechanical engineering and machinery
[SPI.NANO] Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics
Microfluidics
microfluidics
single cell analysis
02 engineering and technology
Capacitive displacement sensor
Article
Displacement (vector)
03 medical and health sciences
[SPI]Engineering Sciences [physics]
[SPI.MECA.BIOM] Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph]
lcsh:TJ1-1570
Electrical measurements
Electrical and Electronic Engineering
[SPI.NANO]Engineering Sciences [physics]/Micro and nanotechnologies/Microelectronics
ComputingMilieux_MISCELLANEOUS
Microelectromechanical systems
[SDV.IB] Life Sciences [q-bio]/Bioengineering
Microchannel
biophysical cell characterization
business.industry
MEMS design
Mechanical Engineering
[SPI.MECA.BIOM]Engineering Sciences [physics]/Mechanics [physics.med-ph]/Biomechanics [physics.med-ph]
021001 nanoscience & nanotechnology
Characterization (materials science)
030104 developmental biology
Control and Systems Engineering
Optoelectronics
[SDV.IB]Life Sciences [q-bio]/Bioengineering
0210 nano-technology
business
Actuator
bioMEMS
Subjects
Details
- Language :
- English
- ISSN :
- 2072666X
- Database :
- OpenAIRE
- Journal :
- Micromachines, Micromachines, MDPI, 2018, 9 (6), pp.275. ⟨10.3390/mi9060275⟩, Micromachines, 2018, 9 (6), pp.275. ⟨10.3390/mi9060275⟩, Micromachines, Vol 9, Iss 6, p 275 (2018), Volume 9, Issue 6
- Accession number :
- edsair.doi.dedup.....c343795f96bba2c4567205de10dd2242