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The endometrium as a novel target for leptin: differences in fertility and subfertility
- Source :
- Molecular Human Reproduction. 6:595-601
- Publication Year :
- 2000
- Publisher :
- Oxford University Press (OUP), 2000.
-
Abstract
- Leptin and its receptor are involved in endocrine and paracrine regulation of metabolism, obesity and reproduction. Here, we describe the detection of the functional long isoform receptor of leptin in human endometrium. The leptin receptor protein was shown to be expressed in glandular and luminal epithelium and is periodically regulated throughout the menstrual cycle, demonstrating main expression in follicular and mid-luteal phase. In contrast, leptin receptor mRNA is detectable by reverse transcription-polymerase chain reaction (RT-PCR) as a constitutive component. Since RT-PCR analyses showed that leptin is not expressed in this tissue, the present study suggests that the human endometrium is a novel target for leptin. Therefore, we investigated 11 subfertile patients who underwent two biopsies in one menstrual cycle. The patients presented with a repetitive endometrial maturation defect, but showed adequate serum hormone concentrations and normal steroid hormone receptor expression and down-regulation in the endometrium. These patients were, however, deficient for expression of the functional leptin receptor. These analyses provide evidence that the lack of the leptin receptor in an ovulatory cycle may contribute to subfertility by a yet undefined 'endometrial factor'.
- Subjects :
- Adult
Leptin
Embryology
medicine.medical_specialty
Steroid hormone receptor
media_common.quotation_subject
Receptors, Cell Surface
Biology
Endometrium
Internal medicine
Follicular phase
Genetics
medicine
Humans
Protein Isoforms
RNA, Messenger
Receptor
Molecular Biology
Menstrual cycle
media_common
Leptin receptor
Reverse Transcriptase Polymerase Chain Reaction
digestive, oral, and skin physiology
Obstetrics and Gynecology
Cell Biology
Immunohistochemistry
Fertility
Endocrinology
medicine.anatomical_structure
Reproductive Medicine
Receptors, Leptin
Female
Carrier Proteins
hormones, hormone substitutes, and hormone antagonists
Developmental Biology
Hormone
Subjects
Details
- ISSN :
- 14602407
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- Molecular Human Reproduction
- Accession number :
- edsair.doi.dedup.....c34d8a235dd51a90f2e82f89de9b9327