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Naked mole rat cells display more efficient excision repair than mouse cells
- Source :
- Aging (Albany NY)
- Publication Year :
- 2018
-
Abstract
- Naked mole rat (NMR) is the long-lived and tumor-resistant rodent. NMRs possess multiple adaptations that may contribute to longevity and cancer-resistance. However, whether NMRs have more efficient DNA repair have not been directly tested. Here we compared base excision repair (BER) and nucleotide excision repair (NER) systems in extracts from NMR and mouse fibroblasts after UVC irradiation. Transcript levels of the key repair enzymes demonstrated in most cases higher inducibility in the mouse vs the NMR cells. Ratios of repair enzymes activities in the extracts somewhat varied depending on post-irradiation time. NMR cell extracts were 2-3-fold more efficient at removing the bulky lesions, 1.5-3-fold more efficient at removing uracil, and about 1.4-fold more efficient at cleaving the AP-site than the mouse cells, while DNA polymerase activities being as a whole higher in the mouse demonstrate different patterns of product distribution. The level of poly(ADP-ribose) synthesis was 1.4-1.8-fold higher in the NMR cells. Furthermore, NMR cell extracts displayed higher binding of PARP1 to DNA probes containing apurinic/apyrimidinic site or photo-reactive DNA lesions. Cumulatively, our results suggest that the NMR has more efficient excision repair systems than the mouse, which may contribute to longevity and cancer resistance of this species.
- Subjects :
- 0301 basic medicine
Aging
Time Factors
DNA Repair
DNA repair
DNA polymerase
Ultraviolet Rays
base excision repair
03 medical and health sciences
chemistry.chemical_compound
Mice
PARP1
Species Specificity
Animals
Mus musculus
AP site
RNA, Messenger
Heterochephalus glaber
030102 biochemistry & molecular biology
biology
Mole Rats
Uracil
Cell Biology
Base excision repair
DNA
Fibroblasts
nucleotide excision repair
Molecular biology
030104 developmental biology
chemistry
Gene Expression Regulation
poly(ADP-ribose) polymerase 1
biology.protein
Nucleotide excision repair
Research Paper
Subjects
Details
- ISSN :
- 19454589
- Volume :
- 10
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Aging
- Accession number :
- edsair.doi.dedup.....c394c319fae11b9aa0d910252b9938a5