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Highly Sensitive PCR Assay for Routine Diagnosis of African Swine Fever Virus in Clinical Samples

Authors :
C. Sánchez Mascaraque
Marisa Arias
Jovita Fernández
Montserrat Agüero
José Manuel Sánchez-Vizcaíno
Luis Romero
Source :
Repositorio de Resultados de Investigación del INIA, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA, INIA: Repositorio de Resultados de Investigación del INIA
Publication Year :
2003
Publisher :
American Society for Microbiology, 2003.

Abstract

This work provides a novel, highly sensitive, hot start PCR method for rapid and specific detection of African swine fever virus (ASFV) that can be used as a routine diagnostic test for ASFV in surveillance, control, and eradication programs. A confirmatory test of the specificity of this method based on restriction endonuclease analysis was also developed.

Details

Language :
English
Database :
OpenAIRE
Journal :
Repositorio de Resultados de Investigación del INIA, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria INIA, INIA: Repositorio de Resultados de Investigación del INIA
Accession number :
edsair.doi.dedup.....c3a487cf0c76deab292d707e0ff5c0de