Supplemental Figure 6 from Inhibition of the EGFR/STAT3/CEBPD Axis Reverses Cisplatin Cross-resistance with Paclitaxel in the Urothelial Carcinoma of the Urinary Bladder

Attenuated pSTAT3, CEBPD, ABCB1 and ABCC2 mRNA levels are observed in CDDP-resistant NTUB1/P xenografted NOD/SCID mice. A and B, Following 3 weeks of drug treatment, all animals were sacrificed, and their tumors were collected for protein and mRNA analysis. Protein samples were employed for Western blot analysis by using specific antibodies. The following data present changes in pSTAT3, CEBPD, and ABCB1 levels in NTUB1/P-xenografted NOD/SCID mice treated with CDDP and gefitinib or with CDDP and S3I-201. qPCR assays were performed using the total RNA harvested from tumor samples. In this figure, *** denotes a significant difference (P < 0.001). B, evaluation of body weight of experimental mice used in Fig. 5. CTL: control.