Comparison of double antigen sandwich and indirect enzyme‐linked immunosorbent assay for the diagnosis of hepatitis C virus antibodies

Background The aim of this study is to compare double‐antigen sandwich enzyme‐linked immunosorbent assay (ELISA) and indirect ELISA in the diagnosis of hepatitis C virus（HCV）infection. Methods and materials A total of 176 samples from the Tumor Hospital Affiliated to Xin Jiang Medical University were utilized to comparison. All serum samples were tested using double‐antigen sandwich ELISA and indirect ELISA. Cohen's kappa statistics were used to assess the agreement between the two assays, and multivariate analysis was used to evaluate risk factors for the discordance between the double‐antigen ELISA and indirect ELISA. Results The positivities of indirect ELISA (Beijing Wantai), double‐antigen sandwich ELISA (Beijing Wantai), and indirect ELISA (Beijing Jinhao) were 74.43%, 68.75%, and 73.30%, respectively. The agreement between the indirect ELISA (Beijing Wantai) and double‐antigen sandwich ELISA (Beijing Wantai) was high (κ = 0.829;P
Compare doubl‐antigen sandwich enzyme‐linked immunosorbent assay (ELISA) and indirect ELISA in the diagnosis of hepatitis C virus (HCV) infection.